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Journal article
Human Serum Enhances Biomimicry of Engineered Tissue Models of Bone and Cancer
Frontiers in bioengineering and biotechnology, v 9, pp 658472-658472
13 Jul 2021
PMID: 34327193
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
For decades, fetal bovine serum (FBS) has been used routinely for culturing many cell types, based on its empirically demonstrated effects on cell growth, and the lack of suitable non-xenogeneic alternatives. The FBS-based culture media do not represent the human physiological conditions, and can compromise biomimicry of preclinical models. To recapitulate
in vitro
the features of human bone and bone cancer, we investigated the effects of human serum and human platelet lysate on modeling osteogenesis, osteoclastogenesis, and bone cancer in two-dimensional (2D) and three-dimensional (3D) settings. For monitoring tumor growth within tissue-engineered bone in a non-destructive fashion, we generated cancer cell lines expressing and secreting luciferase. Culture media containing human serum enhanced osteogenesis and osteoclasts differentiation, and provided a more realistic
in vitro
mimic of human cancer cell proliferation. When human serum was used for building 3D engineered bone, the tissue recapitulated bone homeostasis and response to bisphosphonates observed in native bone. We found disparities in cell behavior and drug responses between the metastatic and primary cancer cells cultured in the bone niche, with the effectiveness of bisphosphonates observed only in metastatic models. Overall, these data support the utility of human serum for bioengineering of bone and bone cancers.
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Details
- Title
- Human Serum Enhances Biomimicry of Engineered Tissue Models of Bone and Cancer
- Creators
- Aranzazu Villasante - Columbia UniversitySamuel T. Robinson - Columbia UniversityAndrew R. Cohen - Drexel UniversityRoberta Lock - Columbia UniversityX. Edward Guo - Columbia UniversityGordana Vunjak-Novakovic - Columbia University
- Publication Details
- Frontiers in bioengineering and biotechnology, v 9, pp 658472-658472
- Publisher
- Frontiers Media S.A
- Grant note
- National Institutes of Health Alternatives Research and Development Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering
- Web of Science ID
- WOS:000679130900001
- Scopus ID
- 2-s2.0-85111360171
- Other Identifier
- 991019296798104721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Engineering, Biomedical