Journal article
Human T cell leukemia virus type 1 Tax induces the expression of murine dendritic cell markers associated with maturation and activation
Journal of neurovirology, Vol.12
01 May 2006
Abstract
HTLV-1 is the etiologic agent of both adult T cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Although the genesis of HAM/TSP likely involves several steps, the generation of a highly specific and effective population of Tax-specific CD8+ cytotoxic T lymphocytes (CTLs) that migrate to the central nervous system is likely of pivotal importance in this neuropathologic process. Presentation of Tax peptides by activated dendritic cells (DCs) to naive CD8+ T cells likely plays an important role in the induction of a Tax-specific CTL response. Studies have suggested that Tax may be secreted from HTLV-1-infected cells and act as an extracellular cytokine. We report herein that treatment of a murine dendritic cell line JAWS II with purified Tax protein induces DC activation and maturation involving an increase in the production of CD80, CD83, and CD86 mRNA as demonstrated by microarray analyses and quantitative RT-PCR assessment. Additionally, treatment of JAWS II DCs with extracellular Tax decreases the ability of DCs to present an MHC class I-restricted peptide, indicating that Tax likely matures the DCs to the point where presentation of a secondary antigen is restricted. The implication of the experimental results is significant with respect to the generation of a Tax-specific CTL compartment and the eventual neurologic dysfunction observed in HAM/TSP.
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Details
- Title
- Human T cell leukemia virus type 1 Tax induces the expression of murine dendritic cell markers associated with maturation and activation
- Creators
- S ManuelK MostollerP JainB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170342404721