Journal article
Human adipose-derived stem cell treatment modulates cellular protection in both in vitro and in vivo traumatic brain injury models
The journal of trauma and acute care surgery, Vol.84(5), pp.745-751
01 May 2018
PMID: 29251705
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
BACKGROUND Traumatic brain injury (TBI) is a common cause of morbidity and mortality in the civilian population. The purpose of this study was to examine the effect(s) of adipose-derived stem cell (ASC) treatment on cellular and functional recovery in TBI via both in vitro and in vivo methods.
METHODS Cultured neuroblastoma cells, SH-SY5Y, were scratched to mimic TBI in an in vitro model. The effect of ASC-conditioned medium (CM) on cell death, mitochondrial function, and expression of inflammatory cytokines (tumor necrosis factor [TNF-], interleukin 1 [IL-1], and IL-6), as well as apoptosis marker FAS, was measured. In our in vivo model, Sprague-Dawley rats underwent TBI via a frontal, closed-head injury model. Animals randomly received either intravenous human-derived ASCs or intravenous saline within 3 hours of injury and were compared with a sham group. Functional recovery was evaluated via accelerating Rotarod method. On post-TBI Day 3, brain tissue was harvested and assessed for cellular damage via enzyme-linked immunosorbent assay for TNF-, as well as immunohistochemical staining for -amyloid precursor protein (-APP).
RESULTS Our in vitro data show that ASC treatment imparted reduced cell death (ratio to control: 1.21 0.066 vs. 1.01 +/- 0.056, p = 0.017), increased cell viability (ratio to control: 0.86 +/- 0.009 vs. 1.09 +/- 0.01, p = 0.0001), increased mitochondrial function (percentage of control: 78 +/- 6% vs. 68 +/- 3%), and significantly decreased levels of inflammatory cytokine IL-1. In our in vivo study, compared with TBI alone, ASC-treated animals showed no difference in functional recovery, lower levels of expressed TNF- (ratio to total protein, 0.47 +/- 0.01 vs. 0.67 +/- 0.04; p < 0.01), and lower levels of -amyloid precursor protein (fluorescence ratio, 0.43 +/- 0.05 vs. 0.69 +/- 0.03; p < 0.01).
CONCLUSIONS Adipose-derived stem cell treatment results in improved cell survival, decreased inflammatory marker release, and decreased evidence of neural injury. No difference in functional recovery was seen. These data suggest the potential for ASC treatment to aid in cellular protection and recovery in neural cells following TBI.
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Details
- Title
- Human adipose-derived stem cell treatment modulates cellular protection in both in vitro and in vivo traumatic brain injury models
- Creators
- Nikolas S. Kappy - Cooper University HospitalShaohua Chang - Cooper University HospitalWilliam M. Harris - Cooper University HospitalMichael Plastini - Cooper University HospitalTelisha Ortiz - Cooper University HospitalPing Zhang - Cooper University HospitalJoshua P. Hazelton - Cooper University HospitalJeffrey P. Carpenter - Cooper University HospitalSpencer A. Brown - Cooper University Hospital
- Publication Details
- The journal of trauma and acute care surgery, Vol.84(5), pp.745-751
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000431415300008
- Scopus ID
- 2-s2.0-85046534779
- Other Identifier
- 991022056902304721
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- Web of Science research areas
- Critical Care Medicine
- Surgery