Journal article
Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70
Cellular immunology, v 266(2), pp 143-153
01 Jan 2011
PMID: 21055733
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
IL-10 transcripts were expressed in 14/15 primary breast adenocarcinomas and in 5/8 established breast tumor lines. Immunohistochemistry and immunoprecipitation from lysates and supernatants revealed that established breast tumor lines produced IL-10 protein. Immunohistochemistry revealed that IL-10 is localized to tumor cells of primary breast adenocarcinomas and to occasional infiltrating MNC. Established breast tumor cell lines expressed IL-12p40 transcripts (6/8) and protein (4/7) and IL-12p35 transcripts (6/7). Using two sandwich ELISAs, specific, respectively, for IL-12p40 and IL-12p70 proteins, we demonstrated that established breast tumor cell lines produce IL-12p40 monomer/homodimer, but not IL-12p70. Positive staining for IL-12p70 in primary breast adenocarcinomas was found only in MNC infiltrating the tumor while tumor cells were negative. IL-12p40 homodimer/monomer inhibit as antagonists IL-12 or IL-23, although they may also act as agonists and positive regulators. Also, primary breast adenocarcinomas (15/15) and established breast tumor cell lines (6/8) expressed TGF-beta 1 transcripts. IL-10, IL-12p40 and TGF-beta 1 may inhibit substantially the anti-tumor immune response. (C) 2010 Elsevier Inc. All rights reserved.
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Details
- Title
- Human breast tumor cells express IL-10 and IL-12p40 transcripts and proteins, but do not produce IL-12p70
- Creators
- Mark C. Heckel - Temple UniversityAlexey Wolfson - Temple UniversityChristopher A. Slachta - Temple UniversityRoland Schwarting - Thomas Jefferson UniversityPadmini Salgame - Temple UniversityChristos D. Katsetos - Temple UniversityChris D. Platsoucas - Temple University
- Publication Details
- Cellular immunology, v 266(2), pp 143-153
- Publisher
- Elsevier
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000286493000006
- Scopus ID
- 2-s2.0-78649988840
- Other Identifier
- 991019353618804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Immunology