Journal article
Human monocytes generate basophil histamine-releasing activities
The Journal of immunology (1950), v 140(7), pp 2369-2375
01 Apr 1988
PMID: 2450919
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human peripheral blood monocytes generated activities during 24-h culture that were capable of triggering histamine release from 17 of 18 human basophil donors. Monocytes and their in vitro transformed macrophages continued to elaborate these basophil histamine-releasing activities for at least 3 wk in culture. In the 18 basophil donors tested, maximum histamine release induced by monocyte supernatants was 33.8 +/- 5.9% (mean +/- SEM) of total basophil histamine content; optimum anti-IgE-induced release was 38.8 +/- 6.2%. Basophil histamine release in response to monocyte activities was optimal at 37 degrees C and at calcium concentrations of 2 to 5 mM. Release was greater than 90% complete 1 min after challenge and was inhibited by anti-allergic drugs. The mechanism of release appeared to be independent of IgE binding. Gel filtration of supernatants derived from both day 1 (monocyte stage) and day 14 (macrophage stage) cultures demonstrated activity peaks with approximate m.w. of 12,000 and 30,000. In contrast to the marked responsiveness of basophils, only 2 of 10 human lung mast cell preparations responded; release in those preparations was low: 3% and 13% histamine release, respectively. Thus, monocytes produce potent histamine-releasing activities with differential actions on basophils and mast cells.
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Details
- Title
- Human monocytes generate basophil histamine-releasing activities
- Creators
- E S Schulman - Department of Medicine, Jefferson Medical College, Philadelphia, PA 19107M C McGettiganT J PostR J VigdermanS S Shapiro
- Publication Details
- The Journal of immunology (1950), v 140(7), pp 2369-2375
- Grant note
- AI-20634 / NIAID NIH HHS HL-09163 / NHLBI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pulmonary, Critical Care, and Sleep (Medicine)
- Web of Science ID
- WOS:A1988M687900038
- Scopus ID
- 2-s2.0-0023894883
- Other Identifier
- 991019295311704721
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- Web of Science research areas
- Immunology