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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Human papillomavirus (HPV) awareness and vaccine receptivity among Senegalese adolescents
Tropical medicine & international health, v 22(1), pp 113-121
01 Jan 2017
PMID: 27754581
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ObjectiveTo examine HPV vaccine awareness and receptivity among adolescents and young adults in Senegal.
MethodsParticipants from six high schools and five community centres across five regions of Senegal (n = 2286) completed a self-administered questionnaire in October and November 2014. The study assessed HPV awareness and receptivity towards receiving the HPV vaccine. Multivariable logistic regression explored statistically significant relationships between the predictor variables and both outcomes.
ResultsTwenty-seven percent had heard of HPV. Among those who had heard of HPV (n = 616), only 28% indicated willingness to vaccinate. Multivariable analysis showed that respondents from rural areas had 63% higher odds (95% CI: 1.24, 2.12) of having heard of HPV than those in urban areas. Respondents with fathers who had completed higher education had 41% higher odds (95% CI: 1.04, 1.92) of being aware of HPV (P < 0.05); however, every level of father's education (as compared to no education at all) was negatively associated with willingness to vaccinate. Respondents who had previously spoken to a healthcare professional about the HPV vaccine had 80% higher odds (95% CI: 1.16, 2.81) of willingness to vaccinate than those who did not speak to a provider about the vaccine.
ConclusionsHealthcare providers and parents are important stakeholders in disseminating HPV vaccine information. Given the overall low levels of awareness, there is a great opportunity for public health communication efforts to craft health messaging and information in a way to maximise receptivity, outlining benefits and providing information on the minimal risks associated with the vaccine.
ObjectifPresenter et evaluer des tests simples et rentables pour determiner la quantite d'insecticide dans les materiaux traites.
MethodesNous avons developpe et evalue un immunoessai competitif sur deux plateformes differentes: un biocapteur impedimetrique sans marqueur (biocapteur EIS) et un flux lateral. Les deux approches ont ete validees par chromatographie en phase gazeuse (GC) et par ELISA, les etalons d'or pour les methodes analytiques et les immunodosages, respectivement. Enfin, des echantillons de moustiquaires impregnees d'insecticide (MII) traitees aux pyrethroides disponibles dans le commerce ont ete analyses. Differentes methodes d'extraction ont ete evaluees.
ResultatsL'extraction des insecticides par infusion directe des echantillons de MII avec du dichloromethane et du dioxane a montre une efficacite de recuperation autour de 100% pour les MII et > 70% pour les moustiquaires incorporees d'insecticide. Ces resultats sont comparables a ceux obtenus avec les methodes de sonication standard. La caracterisation par immunoessai competitif avec l'ELISA presentait une plage dynamique entre 12nM et 1,5M (coefficient de variation (CV) inferieur a 5%), avec une IC50 a 138nM et une limite de detection (LOD) de 3,2nM. Le biocapteur EIS avait une plage lineaire comprise entre 1,7nM et 61nM (CV autour de 14%), avec une IC50 a 10,4nM et une LOD de 0,6nM. Enfin, l'approche par flux lateral a montre une plage dynamique entre 150nM et 1,5M, une IC50 a 505nM et une LOD de 67nM.
ConclusionsL'ELISA peut remplacer la chromatographie comme technique de laboratoire precise pour determiner la concentration d'insecticide dans les moustiquaires. L'approche developpee par flux lateral peut etre utilisee pour estimer la concentration d'insecticide dans les MII sur le terrain. Cette nouvelle technologie, couplee aux nouvelles methodes d'extraction, devrait fournir des directives fiables pour l'utilisation et le remplacement des MII sur le terrain.
ObjetivoPresentar y evaluar pruebas simples y costo-efectivas para determinar la cantidad de insecticida en materiales tratados.
MetodosHemos desarrollado y evaluado un inmunoensayo competitivo en dos plataformas diferentes: un biosensor impedimetrico (biosensor EIS) y uno de flujo lateral. Ambos metodos se validaron utilizando la cromatografia de gases (CG) y ELISA, metodos estandar para pruebas analiticas e inmunoensayos, respectivamente. Finalmente se analizaron muestras de mosquiteras tratadas con piretroides disponibles a nivel comercial. Se evaluaron diferentes metodos de extraccion.
ResultadosLa extraccion de insecticida por infusion directa de las muestras de mosquiteras con diclorometano y dioxano mostraban eficiencias de recuperacion de cerca del 100% para mosquiteras recubiertas de insecticida y de >70% para mosquiteras con insecticida incorporado. Estos resultados eran comparables a aquellos obtenidos con metodos estandar de sonicacion. La caracterizacion del inmunoensayo competitivo con ELISA presentaba un rango dinamico de entre 12nM-1.5M (coeficiente de variacion (CV) por debajo del 5%), con un IC50 a 138nM, y un limite de deteccion (LdD) de 3.2nM. El biosensor EIS tenia un rango linear entre 1.7nM-61nM (CV cerca del 14%), con un IC50 a 10.4nM, y un LdD de 0.6nM. Finalmente, la metodologia de flujo lateral mostro un rango dinamico de entre 150nM-1.5M, un IC50 a 505nM, y un LdD de 67nM.
ConclusionesLos ELISA pueden sustituir la cromatografia como una tecnica de laboratorio precisa para determinar la concentracion de insecticida en mosquiteras. La prueba de flujo lateral puede utilizarse para calcular la concentracion de insecticida en mosquiteras estando en el campo. Esta nueva tecnologia, junto con los nuevos metodos de extraccion, deberia proporcionar guias fiables para el uso de mosquiteras y su reemplazo en el campo.
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Details
- Title
- Human papillomavirus (HPV) awareness and vaccine receptivity among Senegalese adolescents
- Creators
- Philip M. Massey - Philadelphia Department of Public HealthRuth K. Boansi - Philadelphia Department of Public HealthJessica D. Gipson - Department of Community Health Sciences, UCLA Fielding School of Public Health, Los Angeles, CA, USA,Rachel M. Adams - Department of Community Health Sciences, UCLA Fielding School of Public Health, Los Angeles, CA, USA,Helene Riess - Department of Community Health Sciences, UCLA Fielding School of Public Health, Los Angeles, CA, USA,Thierno Dieng - Hôpital Aristide Le DantecMichael L. Prelip - Department of Community Health Sciences, UCLA Fielding School of Public Health, Los Angeles, CA, USA,Deborah C. Glik - Department of Community Health Sciences, UCLA Fielding School of Public Health, Los Angeles, CA, USA,
- Publication Details
- Tropical medicine & international health, v 22(1), pp 113-121
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- K01HD067677 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) R03TW009043 / FOGARTY INTERNATIONAL CENTER; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Fogarty International Center (FIC) TW009043-01 / NIH-NICHD Fogarty Behavioral and Social Science
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Community Health and Prevention
- Web of Science ID
- WOS:000392528900012
- Scopus ID
- 2-s2.0-85003727782
- Other Identifier
- 991019167795604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine