Journal article
Hydrogel encapsulation to improve cell viability during syringe needle flow
Journal of long-term effects of medical implants, v 24(2-3), pp 151-162
2014
PMID: 25272214
Abstract
This work examines pluronic F-127 poloxamer for cell protection during injection through a syringe needle. Direct cell injection is a minimally invasive method for cell transplantation; however, it often results in poor cell viability. We proposed that encapsulating cells in this hydrogel would protect cells from detrimental mechanical forces during injection and increase cell viability. The hydrogel was tested at multiple weights and carbon nanobrush concentrations to determine how gel weight affects cell viability as well as to allow the gels to remain as electrically conductive scaffolds. This work assessed the ability of the hydrogel to prevent cell membrane bursting. We used D1 multipotent mouse bone marrow stromal precursor cells for this study. We found that the pressure drop increases with increasing weight of the gels. However, cell viability also increases as the weight of the gels increases. These results support the proposition that hydrogels can be used to protect cells during syringe needle injection. Since these hydrogels undergo a reverse phase transition, the gels can be used to transplant cells into the body in solution form through injection. The gels will then harden in situ to allow for cell proliferation and tissue regeneration at the desired site.
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15 citations in Scopus
Details
- Title
- Hydrogel encapsulation to improve cell viability during syringe needle flow
- Creators
- Matthew A Wagner - Harvard UniversityWilliam H Marks - Harvard UniversitySujata K Bhatia - Harvard University
- Publication Details
- Journal of long-term effects of medical implants, v 24(2-3), pp 151-162
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Scopus ID
- 2-s2.0-84906495239
- Other Identifier
- 991021230003404721