Journal article
Hyperbranched lysine−arginine copolymer for gene delivery
Journal of biomaterials science. Polymer ed, v 26(16), pp 1163-1177
02 Nov 2015
PMID: 26249038
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Based on the reactivity of amine groups and carboxyl groups of L-lysine and L-arginine, thermal polymerization of these two natural amino acids results in hyperbranched lysine-arginine copolymers (P-lys-arg
X
, where X refers to the relevant molar ratio of arginine to lysine). Hyperbranched polylysine (P-lys) and two derivatives (P-lys-arg
0.10
and P-lys-arg
0.20
) have been prepared. The arginine-rich hyperbranched polymers can interact with plasmid DNA to form nano-sized particles. The polyplexes were physicochemically analyzed by agarose gel electrophoresis, dynamic light scattering, and zeta potential measurements. Furthermore, their transfection efficiency was assessed, employing COS-7, 293T, and HeLa cell lines. It was found that P-lys showed poorly in its ability of condensation with DNA and transfection efficiency. On the other hand, arginine-rich products resulted to significant enhancement of its transfection efficiency, which is dependent on the content of arginine in the polymers, and the cell line used. P-lys-arg
0.20
exhibited better transfection efficiency under all the condition studied. Besides, P-lys-arg
0.20
showed lower toxicity in COS-7 cells.
Metrics
Details
- Title
- Hyperbranched lysine−arginine copolymer for gene delivery
- Creators
- Qi Peng - Xinyang Normal UniversityJianjun Zhu - Xinyang Normal UniversityYongsheng Yu - Xinyang Normal UniversityLee Hoffman - Drexel UniversityXingkun Yang - Xinyang Normal University
- Publication Details
- Journal of biomaterials science. Polymer ed, v 26(16), pp 1163-1177
- Publisher
- Taylor & Francis
- Grant note
- 12B150026 / The Education the Education Department of Henan Province of China 2013GGJS-123 / Fundation for University Young Key Teacher of He'nan Educational Committee
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000370288800007
- Scopus ID
- 2-s2.0-84942815641
- Other Identifier
- 991019168662304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- Polymer Science