Journal article
Hyperoxia causes miR199a-5p-mediated injury in the developing lung
Pediatric research, v 86(5), pp 579-588
01 Nov 2019
PMID: 31390652
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
BACKGROUND: Hyperoxia-induced acute lung injury (HALI) is characterized by increased permeability and infiltration of inflammatory cells, impairment of alveolar development, and compromised lung function. Recent evidence has determined that microRNAs (miRs) are implicated in hyperoxia-induced lung injury, including bronchopulmonary dysplasia (BPD). However, the expression profile and functional role of miR199a-5p in developing lungs have not been reported.
METHODS: The present study was undertaken to explore the role of miR199a-5p in developing mice lungs and human neonates. We exposed neonatal mice for 7 days, mouse lung epithelial cells (MLE12), mouse lung endothelial cells (MLECs), and macrophages (RAW246.7), to hyperoxia at different time points.
RESULTS: Our results demonstrated enhanced miR199a-5p expression in hyperoxia-exposed mice lungs and cells, as well as in tracheal aspirates of infants developing BPD, with significant reduction in the expression of its target, caveolin-1. Next, we observed that miR199a-5p-mimic worsens HALI as evidenced by increased inflammatory cells, cytokines, and lung vascular markers. Conversely, miR199a-5p-inhibitor treatment attenuated HALI.
CONCLUSION: Thus, our findings suggest that miR199a-5p is a potential target for attenuating HALI pathophysiology in the developing lung. Moreover, miR199a-5p-inhibitor could be part of a novel therapeutic strategy for improving BPD in preterm neonates.
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Details
- Title
- Hyperoxia causes miR199a-5p-mediated injury in the developing lung
- Creators
- Mohammad Afaque Alam - Temple UniversitySuhita Gayen Nee Betal - Thomas Jefferson Univ, Dept Pediat, Div Neonatol, Philadelphia, PA USAZubair H. Aghai - Thomas Jefferson UniversityVineet Bhandari - Drexel University
- Publication Details
- Pediatric research, v 86(5), pp 579-588
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- Departmental Funds
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000496506400010
- Scopus ID
- 2-s2.0-85071433731
- Other Identifier
- 991019167639104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Pediatrics