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Journal article
Hypothalamic injection of non-opioid peptides increases gene expression of the opioid enkephalin in hypothalamic and mesolimbic nuclei: Possible mechanism underlying their behavioral effects
Peptides (New York, N.Y. : 1980), Vol.30(12), pp.2423-2431
Dec 2009
PMCID: PMC2787664
PMID: 19782113
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Abstract
The peptides galanin (GAL) and orexin (OX) share common features with the opioid enkephalin (ENK) in their relationship to ingestive behavior, stimulating consumption of a fat-rich diet and ethanol when injected into the hypothalamus. Since receptors for GAL and OX are dense in areas where ENK-expressing neurons are concentrated, these non-opioid peptides may exert their effects, in part, through the stimulation of endogenous ENK. This study was conducted to determine whether injection of GAL or OX affects the expression of ENK in hypothalamic and mesolimbic nuclei involved in consummatory behavior. Rats were injected with GAL (1 microg), OX-A (1 microg), or saline vehicle just dorsal to the hypothalamic paraventricular nucleus (PVN). They were sacrificed 1h later for analysis of ENK mRNA levels in the PVN, ventral tegmental area (VTA), central nucleus of the amygdala (CeA), and nucleus accumbens (NAc). Both GAL and OX had similar effects, significantly increasing ENK mRNA expression in each of these areas, except for the NAc. This enhanced ENK expression in the PVN, VTA and CeA was demonstrated with real-time quantitative polymerase chain reaction and confirmed in separate groups using radiolabeled and digoxigenin-labeled in situ hybridization. These findings demonstrate that the non-opioid peptides, GAL or OX, which have similar effects on consummatory behavior, are also similar in their effect on endogenous ENK. In light of published findings showing an opioid antagonist to block GAL- and OX-induced feeding, these results provide additional evidence that ENK is involved in mediating the common behavioral effects of these peptides.
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Details
- Title
- Hypothalamic injection of non-opioid peptides increases gene expression of the opioid enkephalin in hypothalamic and mesolimbic nuclei: Possible mechanism underlying their behavioral effects
- Creators
- Olga Karatayev - Rockefeller UniversityJessica R Barson - Rockefeller UniversityGuo-Qing ChangSarah F Leibowitz - Rockefeller University
- Publication Details
- Peptides (New York, N.Y. : 1980), Vol.30(12), pp.2423-2431
- Publisher
- Elsevier
- Grant note
- R01 AA012882 / NIAAA NIH HHS AA12882 / NIAAA NIH HHS R01 DA021518 / NIDA NIH HHS DA21518 / NIDA NIH HHS R01 DA021518-18 / NIDA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Neurobiology and Anatomy; Drexel University
- Identifiers
- 991020100070704721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism
- Pharmacology & Pharmacy