Journal article
Hypoxia modifies nuclear calcium uptake pathways in the cerebral cortex of the guinea-pig fetus
Neuroscience, v 130(4), pp 949-955
2005
PMID: 15652992
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nuclear Ca
2+ signals are thought to play a critical role in the initiation and progression of programmed cell death. The present study tests the hypothesis that hypoxia alters nuclear Ca
2+ transport pathways and leads to an increase in nuclear Ca
2+-influx in cerebral cortical neuronal nuclei. To test this hypothesis the effect of tissue hypoxia on high affinity Ca
2+-ATPase activity and the binding characteristics of inositol 1,4,5-triphosphate (IP
3) and inositol 1,3,4,5-tetrakisphosphate (IP
4) receptors were studied in neuronal nuclei from the cerebral cortex of guinea-pig fetuses. Results show increased high-affinity Ca
2+-ATPase activity (nmol/mg protein/h) in the hypoxic group 969.7±79 as compared with 602.4±90.9 in the normoxic group,
P<0.05. The number of IP
3 receptors (Bmax, fmol/mg protein) increased from 61±21 in the normoxic group to 164±49 in the hypoxic group,
P<0.05.
K
d values did not change following hypoxia. In contrast, IP
4 receptor Bmax (fmol/mg protein) and
K
d (nM) values increased from 360±32 in the normoxic group to 626±136 in the hypoxic group (
P<0.001) and, from 26±1 in the normoxic group to 61±9 in the hypoxic group (
P<0.001), respectively.
45Ca
2+-influx (pmol/mg protein) significantly increased from 6.3±1.9 in the normoxic group to 10.9±1.1 the hypoxic group (
P<0.001). The data show that hypoxia modifies nuclear Ca
2+ transport pathways and results in increased nuclear Ca
2+-influx. We speculate that hypoxia increases nuclear Ca
2+ uptake from the cytoplasm to the nucleoplasm, resulting in increased transcription of proapoptotic genes and subsequent activation of programmed cell death pathways.
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Details
- Title
- Hypoxia modifies nuclear calcium uptake pathways in the cerebral cortex of the guinea-pig fetus
- Creators
- S.A. Zanelli - University of VirginiaE. Spandou - Aristotle University of ThessalonikiO.P. Mishra - St. Christopher's Hospital for ChildrenM. Delivoria-Papadopoulos - St. Christopher's Hospital for Children
- Publication Details
- Neuroscience, v 130(4), pp 949-955
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000226766000013
- Scopus ID
- 2-s2.0-11844284814
- Other Identifier
- 991019168151204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences