Journal article
IDENTIFICATION OF THE RECEPTOR FOR A NOVEL M(R)78000 INVASION STIMULATING FACTOR FROM METASTATIC HUMAN PROSTATIC PC-3 ML CLONES
Cancer research (Chicago, Ill.), v 54(9), pp 2492-2495
01 May 1994
PMID: 8162599
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A M(r) 78,000 protein (reduced), termed invasion stimulating factor (ISF), was purified from the conditioned medium of a bone metastasizing human prostatic PC-3 ML clone (M. E. Stearns and M. Stearns. Cancer Metastasis Rev., 12: 39-52, 1993). Scatchard analysis and affinity crosslinking studies revealed that the producer PC-3 hit cells expressed a receptor binding site (M(r) approximate to 115,000). We found a K-d approximate to 425 pM and about 22,000 sites/cell. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiograms confirmed that the PC-3 ML cells expressed the receptor, whereas the ISF non-producing, noninvasive PC-3 clones (i.e., 3-4 x N.I. PC-3 cells) failed to express the ISF receptor. We conclude that a unique ISF autocrine loop characterizes the bone metastatic PC-3 ML cells.
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Details
- Title
- IDENTIFICATION OF THE RECEPTOR FOR A NOVEL M(R)78000 INVASION STIMULATING FACTOR FROM METASTATIC HUMAN PROSTATIC PC-3 ML CLONES
- Creators
- M WangM StearnsM E Stearns
- Publication Details
- Cancer research (Chicago, Ill.), v 54(9), pp 2492-2495
- Publisher
- Amer Assoc Cancer Research
- Number of pages
- 4
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Decision Sciences (and Management Information Systems)
- Web of Science ID
- WOS:A1994NH25600038
- Scopus ID
- 2-s2.0-0028260047
- Other Identifier
- 991019184201004721
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- Web of Science research areas
- Oncology