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Journal article
INTS6/DICE1 inhibits growth of human androgen-independent prostate cancer cells by altering the cell cycle profile and Wnt signaling
Cancer cell international, v 9(1), pp 28-28
11 Nov 2009
PMID: 19906297
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: The gene encoding integrator complex subunit 6 (INTS6), previously known as deleted in cancer cells 1 (DICE1, OMIM 604331) was found to be frequently affected by allelic deletion and promoter hypermethylation in prostate cancer specimens and cell lines. A missense mutation has been detected in prostate cancer cell line LNCaP. Together, these results suggest INTS6/DICE1 as a putative tumor suppressor gene in prostate cancer. In this study, we examined the growth inhibitory effects of INTS6/DICE1 on prostate cancer cells.
Results: Markedly decreased INTS6/DICE1 mRNA levels were detected in prostate cancer cell lines LNCaP, DU145 and PC3 as well as CPTX1532 as compared to a cell line derived from normal prostate tissue, NPTX1532. Exogenous re-expression of INTS6/DICE1 cDNA in androgen-independent PC3 and DU145 cell lines substantially suppressed their ability to form colonies in vitro. This growth inhibition was not due to immediate induction of apoptosis. Rather, prostate cancer cells arrested in G1 phase of the cell cycle. Expression profiling of members of the Wnt signaling pathway revealed up-regulation of several genes including disheveled inhibitor CXXC finger 4 (CXXC4), frizzled homologue 7 (FZD7), transcription factor 7-like 1 (TCF7L1), and down-regulation of cyclin D1.
Conclusion: These results show for the first time a link between INTS6/DICE1 function, cell cycle regulation and cell-cell communication involving members of the Wnt signaling pathway.
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- Title
- INTS6/DICE1 inhibits growth of human androgen-independent prostate cancer cells by altering the cell cycle profile and Wnt signaling
- Creators
- Stephanie Filleur - Texas Tech University Health Sciences CenterJennifer Hirsch - Texas Tech University Health Sciences CenterAline Wille - Otto-von-Guericke University MagdeburgMargarete Schoen - Univ Wurzburg, DFG Res Ctr Expt Biomed, Rudolf Virchow Ctr, Wurzburg, GermanyChristian Sell - Drexel UniversityMichael H. Shearer - Texas Tech University Health Sciences CenterThomas Nelius - Texas Tech University Health Sciences CenterIlse Wieland - Otto-von-Guericke University Magdeburg
- Publication Details
- Cancer cell international, v 9(1), pp 28-28
- Publisher
- Springer Nature
- Number of pages
- 9
- Grant note
- South Plains Foundation, Lubbock, TX
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000273391000001
- Scopus ID
- 2-s2.0-71049118228
- Other Identifier
- 991019169320404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology