Journal article
Identification and characterization of C/EBP binding sites within the HIV-1 subtype C LTR
Journal of neurovirology, Vol.13, pp.109-109
01 Jan 2007
Abstract
Human immunodeficiency virus type 1 (HIV-1) has been transmitted worldwide and regional viral subtypes have been designated as subtype A through K. Subtype C, which is concentrated in Southeast Asia and sub-Saharan Africa, is the most prevalent subtype worldwide. To date, few studies have examined the role of CCAAT/enhancer binding proteins (C/EBP) with respect to long terminal repeat (LTR)-directed viral gene expression from the subtype C LTR. Within subtype B viruses, two functional C/EBP sites upstream of the TATA box have been shown to be required for efficient viral replication in cells of monocyte-macrophage lineage, in order to assess the roles of C/EBP sites within the subtype C viral LTR, 211 HIV-1 subtype C LTR sequences were collected and aligned via the Clustal V method. From these analyses, three putative C/EBP binding sites were identified: two upstream binding sites 1 and 2 (US1 and US2) and one downstream binding site (DS1). Interestingly, the putative downstream site (DS1) is highly conserved between subtypes B and C, suggesting the presence of a functionally important cis-acting element that has yet to be characterized. Electrophoretic mobility shift analysis demonstrated that two of three sites within the HIV-1 subtype C were able to bind C/EBP factors (US1 and DS1). Additional studies focused on examining relative binding affinities of naturally occurring variants of these two sites are currently underway. Future studies will examine the molecular architecture of these sites relative to the functional properties of the HIV-1 LTR.
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Details
- Title
- Identification and characterization of C/EBP binding sites within the HIV-1 subtype C LTR
- Creators
- M NonnemacherY LiuE KilareskiL LiuB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.13, pp.109-109
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170382504721