Journal article
Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos
Developmental biology, v 149(2), pp 357-369
Feb 1992
PMID: 1730390
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Abstract
Sequence analysis of cDNA clones encoding fibronectin (FN) from
Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternatively spliced V region of the protein. These cDNAs have been used to study the expression of FN mRNAs during early development. Overall, levels of maternal FN mRNA remain constant until the mid- to late-gastrula stage when the accumulation of new FN transcripts is first apparent. RNase protection analyses reveal that the pattern of FN alternative splicing is similar to that reported for other species and does not change with the shift from maternal to zygotic mRNA expression. The cellular forms of the FN protein predominate in the early embryo with the EIIIA and EIIIB exons included in most mRNAs at this time. A comparison of V-region alternative splicing between embryonic and adult liver RNAs indicates a segment of 345 nucleotides that can be either completely excluded or included in mature FN transcripts but there is no evidence for additional V-region variants. Maternal mRNAs encoding alternatively spliced forms of FN can be specifically eliminated from
Xenopus oocytes following the injection of antisense oligodeoxynucleotides into the cytoplasm, thereby making it possible to analyze the structure, composition, and function of FN mRNAs in early embryos.
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Details
- Title
- Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos
- Creators
- Douglas W. DeSimone - University of VirginiaPamela A. Norton - Massachusetts Institute of TechnologyRichard O. Hynes - Massachusetts Institute of Technology
- Publication Details
- Developmental biology, v 149(2), pp 357-369
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1992HB61900011
- Scopus ID
- 2-s2.0-0026564757
- Other Identifier
- 991020830155404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Developmental Biology