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Journal article
Identification of Cj1051c as a Major Determinant for the Restriction Barrier of Campylobacter jejuni Strain NCTC11168
Applied and environmental microbiology, v 78(22), pp 7841-7848
01 Nov 2012
PMID: 22923403
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Campylobacter jejuni
is a leading cause of human diarrheal illness in the world, and research on it has benefitted greatly by the completion of several genome sequences and the development of molecular biology tools. However, many hurdles remain for a full understanding of this unique bacterial pathogen. One of the most commonly used strains for genetic work with
C. jejuni
is NCTC11168. While this strain is readily transformable with DNA for genomic recombination, transformation with plasmids is problematic. In this study, we have identified a determinant of this to be
cj1051c
, predicted to encode a restriction-modification type IIG enzyme. Knockout mutagenesis of this gene resulted in a strain with a 1,000-fold-enhanced transformation efficiency with a plasmid purified from a
C. jejuni
host. Additionally, this mutation conferred the ability to be transformed by plasmids isolated from an
Escherichia coli
host. Sequence analysis suggested a high level of variability of the specificity domain between strains and that this gene may be subject to phase variation. We provide evidence that
cj1051c
is active in NCTC11168 and behaves as expected for a type IIG enzyme. The identification of this determinant provides a greater understanding of the molecular biology of
C. jejuni
as well as a tool for plasmid work with strain NCTC11168.
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Details
- Title
- Identification of Cj1051c as a Major Determinant for the Restriction Barrier of Campylobacter jejuni Strain NCTC11168
- Creators
- Jeffrey P. Holt - Drexel UniversityAndrew J. Grant - University of CambridgeChristopher Coward - Department of Veterinary Medicine, University of Cambridge, Cambridge, United KingdomDuncan J. Maskell - University of CambridgeJennifer J. Quinlan - Drexel University
- Publication Details
- Applied and environmental microbiology, v 78(22), pp 7841-7848
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Nutrition Sciences
- Web of Science ID
- WOS:000310427500004
- Scopus ID
- 2-s2.0-84868624319
- Other Identifier
- 991019168134104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology