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Journal article
Identification of Five Interferon-Induced Cellular Proteins That Inhibit West Nile Virus and Dengue Virus Infections
Journal of virology, v 84(16), pp 8332-8341
09 Jun 2010
PMID: 20534863
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Interferons (IFNs) are key mediators of the host innate antiviral immune response. To identify IFN-stimulated genes (ISGs) that instigate an antiviral state against two medically important flaviviruses, West Nile virus (WNV) and dengue virus (DENV), we tested 36 ISGs that are commonly induced by IFN-α for antiviral activity against the two viruses. We discovered that five ISGs efficiently suppressed WNV and/or DENV infection when they were individually expressed in HEK293 cells. Mechanistic analyses revealed that two structurally related cell plasma membrane proteins, IFITM2 and IFITM3, disrupted early steps (entry and/or uncoating) of the viral infection. In contrast, three IFN-induced cellular enzymes, viperin, ISG20, and double-stranded-RNA-activated protein kinase, inhibited steps in viral proteins and/or RNA biosynthesis. Our results thus imply that the antiviral activity of IFN-α is collectively mediated by a panel of ISGs that disrupt multiple steps of the DENV and WNV life cycles.
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Details
- Title
- Identification of Five Interferon-Induced Cellular Proteins That Inhibit West Nile Virus and Dengue Virus Infections
- Creators
- Dong Jiang - Drexel UniversityJessica M. Weidner - Drexel UniversityMin Qing - NovartisXiao-Ben Pan - Drexel UniversityHaitao Guo - Drexel UniversityChunxiao Xu - Drexel UniversityXianchao Zhang - Drexel UniversityAlex Birk - Hepatitis B FoundationJinhong Chang - Drexel UniversityPei-Yong Shi - NovartisTimothy M. Block - Drexel UniversityJu-Tao Guo - Drexel University
- Publication Details
- Journal of virology, v 84(16), pp 8332-8341
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000279983200041
- Scopus ID
- 2-s2.0-77954983994
- Other Identifier
- 991019169602004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology