Journal article
Identification of Global Alteration of Translational Regulation in Glioma In Vivo
PloS one, v 7(10), pp e46965-e46965
03 Oct 2012
PMID: 23056544
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Post-transcriptional regulation of gene expression contributes to the protein output of a cell, however, methods for measuring translational regulation in complex in vivo systems are lacking. Here, we describe a sensitive method for measuring translational regulation in defined cell populations from heterogeneous tissue in vivo. We adapted the translating ribosome affinity purification (TRAP) methodology to measure the relative occupancy of individual mRNA transcripts in translating ribosomes in the Olig2-positive tumor cell population in a genetically engineered mouse model (GEM) of glioma. Global measurement of paired ribosome-bound and total cellular mRNA populations from tumor cells in vivo identified a broad distribution of relative ribosome occupancies amongst mRNA species that was highly reproducible across biological samples. Comparison of the translation state of glioma cells to non-transformed oligodendrocyte progenitor cells in normal brain identified global alteration of translation in tumor, and specifically of genes involved in cell division and synthetic metabolism. Furthermore, investigation of alteration in steady state translational efficiencies upon loss of PTEN, one of the most frequently mutated and deleted tumor suppressors in glioma, identified differential translation of proteins involved in cellular respiration, canonically regulated by PI3K/Akt signaling, and cellular glycosylation profiles, deregulation of which is known to be associated with tumor progression. Application of the translation efficiency profiling method described here to other biological contexts and conditions would extend our knowledge of the scope and impact of this important mode of gene regulation in complex in vivo systems.
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Details
- Title
- Identification of Global Alteration of Translational Regulation in Glioma In Vivo
- Creators
- Karim Helmy - Memorial Sloan Kettering Cancer CenterJohn Halliday - Memorial Sloan Kettering Cancer CenterElena Fomchenko - Memorial Sloan Kettering Cancer CenterManu Setty - Memorial Sloan Kettering Cancer CenterKen Pitter - Mécanique et Engrenage ModerneChristoph Hafemeister - New York UniversityEric C. Holland - Memorial Sloan Kettering Cancer Center
- Publication Details
- PloS one, v 7(10), pp e46965-e46965
- Publisher
- Public Library Science
- Number of pages
- 11
- Grant note
- RO1 CA100688; U54 CA126518; U01 CA141502-01; U54 CA143798 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology
- Web of Science ID
- WOS:000309454000105
- Scopus ID
- 2-s2.0-84867095368
- Other Identifier
- 991022004878604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology