Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Identification of a Stable Flavin-thiolate Adduct in Heterotetrameric Sarcosine Oxidase
Journal of molecular biology, v 362(4), pp 656-663
2006
PMID: 16934831
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Heterotetrameric sarcosine oxidase (TSOX) is a complex bifunctional flavoenzyme that contains two flavins. Most of the FMN in recombinant TSOX is present as a covalent adduct with an endogenous ligand. Enzyme denaturation disrupts the adduct, accompanied by release of a stoichiometric amount of sulfide. Enzyme containing ≥
90% unmodified FMN is prepared by displacement of the endogenous ligand with sulfite, a less tightly bound competing ligand. Reaction of adduct-depleted TSOX with sodium sulfide produces a stable complex that resembles the endogenous TSOX adduct and known 4a-S-cysteinyl flavin adducts. The results provide definitive evidence for sulfide as the endogenous TSOX ligand and strongly suggest that the modified FMN is a 4a-sulfide adduct. A comparable reaction with sodium sulfide is not detected with other flavoprotein oxidases. A model of the postulated TSOX adduct suggests that it is stabilized by nearby residues that may be important in the electron transferase/oxidase function of the coenzyme.
Metrics
Details
- Title
- Identification of a Stable Flavin-thiolate Adduct in Heterotetrameric Sarcosine Oxidase
- Creators
- Robert M.G. Hynson - Drexel UniversityF. Scott Mathews - Washington University in St. LouisMarilyn Schuman Jorns - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USAMarilyn S Jorns - [Retired Faculty]
- Publication Details
- Journal of molecular biology, v 362(4), pp 656-663
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000241324700003
- Scopus ID
- 2-s2.0-33748320783
- Other Identifier
- 991019168840204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology