Journal article
Identification of a Survival-Promoting Peptide in Medium Conditioned by Oxidatively Stressed Cell Lines of Nervous System Origin
The Journal of neuroscience, v 18(18), pp 7047-7060
15 Sep 1998
PMID: 9736629
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A survival-promoting peptide has been purified from medium conditioned by Y79 human retinoblastoma cells and a mouse hippocampal cell line (HN 33.1) exposed to H
2
O
2
. A 30 residue synthetic peptide was made on the basis of N-terminal sequences obtained during purification, and it was found to exhibit gel mobility and staining properties similar to the purified molecules. The peptide maintains cells and their processes
in vitro
for the HN 33.1 cell line treated with H
2
O
2
, and
in vivo
for cortical neurons after lesions of the cerebral cortex. It has weak homology with a fragment of a putative bacterial antigen and, like that molecule, binds IgG. The peptide also contains a motif reminiscent of a critical sequence in the catalytic region of calcineurin-type phosphatases; surprisingly, like several members of this family, the peptide catalyzes the hydrolysis of
para
-nitrophenylphosphate in the presence of Mn
2+
. Application of the peptide to one side of bilateral cerebral cortex lesions centered on area 2 in rats results in an increase in IgG immunoreactivity in the vicinity of the lesions 7 d after surgery. Microglia immunopositive for IgG and ED-1 are, however, dramatically reduced around the lesions in the treated hemisphere. Furthermore, pyramidal neurons that would normally shrink, die, or disintegrate were maintained, as determined by MAP2 immunocytochemistry and Nissl staining. These survival effects were often found in both hemispheres. The results suggest that this peptide operates by diffusion to regulate the immune response and thereby rescue neurons that would usually degenerate after cortical lesions. The phosphatase activity of this molecule also suggests the potential for direct neuron survival-promoting effects.
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Details
- Title
- Identification of a Survival-Promoting Peptide in Medium Conditioned by Oxidatively Stressed Cell Lines of Nervous System Origin
- Creators
- Timothy J CunninghamLisa HodgeDavid SpeicherDave ReimCarla Tyler-PolszPat LevittKathie EaglesonSarah KennedyYing Wang
- Publication Details
- The Journal of neuroscience, v 18(18), pp 7047-7060
- Publisher
- Society for Neuroscience
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000075893000002
- Scopus ID
- 2-s2.0-0032530752
- Other Identifier
- 991014878643204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences