Journal article
Identification of a new murine eosinophil major basic protein (mMBP) gene: cloning and characterization of mMBP‐2
Journal of leukocyte biology, v 67(4), pp 567-576
Apr 2000
PMID: 10770291
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have identified a new eosinophil major basic protein gene family member in the mouse and have given it the designation murine major basic protein‐2 (mMBP‐2). The gene was initially characterized as a unique expressed sequence tag (EST) clone having significant identity to the previously recognized member of this gene family, mMBP‐1. The EST was used to screen and isolate mMBP‐2 from a bone marrow cDNA library. In addition, a genomic clone of mMBP‐2 was isolated and this gene was shown to be physically linked to within 100 kb of mMBP‐1 on the central region of mouse chromosome 2. Progressive similarity alignment of the deduced mMBP‐2 open reading frame demonstrates the apparent conservation of the “pre‐pro‐mature” protein structure found in the other known mammalian MBPs. Mature mMBP‐2 maintains the cationic nature associated with these proteins with a predicted pI of 9.95. However, unlike the human MBPs, which display a three orders of magnitude charge difference [hMBP‐1 (pI11.4) vs. hMBP‐2 (pI8.7)], mMBP‐2 is only slightly less cationic than mMBP‐1 (pI 10.5). Expression studies demonstrate that transcription of the mMBP‐2 gene parallels mMBP‐1 and is confined to hematopoietic compartments engaged in eosinophilopoiesis. Moreover, using mMBP‐1 knockout mice and immunohistochemistry with an antisera that recognizes both mMBP‐1 and ‐2, we demonstrate that mMBP‐2 protein expression is restricted to eosinophil lineage‐committed cells. J. Leukoc. Biol. 67: 567–576; 2000.
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Details
- Title
- Identification of a new murine eosinophil major basic protein (mMBP) gene: cloning and characterization of mMBP‐2
- Creators
- MiMi P MaciasKenneth C WelchKaren L DenzlerKirsten A LarsonNancy A LeeJames J Lee
- Publication Details
- Journal of leukocyte biology, v 67(4), pp 567-576
- Publisher
- Wiley
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000087964900018
- Scopus ID
- 2-s2.0-0033855829
- Other Identifier
- 991014878165304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Hematology
- Immunology