Logo image
Back
Identification of a viral kinase that phosphorylates specific E2Fs and pocket proteins
Journal article   Open access   Peer reviewed

Identification of a viral kinase that phosphorylates specific E2Fs and pocket proteins

S Pajovic, E L Wong, A R Black and J C Azizkhan
Molecular and cellular biology, v 17(11), pp 6459-6464
Nov 1997
DOI: https://doi.org/10.1128/MCB.17.11.6459
PMID: 9343408
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1128/MCB.17.11.6459View
Published, Version of Record (VoR) Open

Abstract

Protein Kinases - metabolism Amino Acid Sequence Phosphorylation E2F1 Transcription Factor Molecular Sequence Data Substrate Specificity E2F Transcription Factors Nuclear Proteins - metabolism Viral Proteins Phosphoproteins - metabolism Proteins Transcription Factor DP1 Transcription Factors - metabolism Immediate-Early Proteins - metabolism Retinoblastoma-Like Protein p130 Transcription, Genetic Cytomegalovirus - enzymology Retinoblastoma-Binding Protein 1 Cell Cycle Proteins Carrier Proteins DNA-Binding Proteins
The transcription factor E2F and its regulation by pRB and related pocket proteins are central to cell cycle control in higher eukaryotes. Much of our knowledge of this regulation has come from studies using immediate-early proteins of DNA tumor viruses. Previously, we reported that the 72-kDa immediate-early region 1 gene product of the human cytomegalovirus, IE72, transactivates the dihydrofolate reductase promoter through the E2F site and that it physically interacts with E2F1 (M. J. Margolis, S. Pajovic, E. L. Wong, M. Wade, R. Jupp, J. A. Nelson, and J. C. Azizkhan, J. Virol. 69:7759-7767, 1995). In this study, we further characterized the mechanism by which IE72 modulates E2F-dependent transcription. In vitro phosphorylation reactions using gel-purified bacterially expressed proteins revealed that IE72 is a kinase that autophosphorylates and phosphorylates E2F1, -2, and -3 (but not E2F4 or -5) and the RB-related pocket proteins p130 and p107 (but not pRB). The region of IE72 spanning amino acids 173 to 197 shows a high level of homology to the ATP binding sites in over 500 kinases. The kinase-negative protein IE72deltaATP, from which this region has been deleted, cannot activate E2F-dependent transcription. The kinase activity of IE72 is also required for its ability to reduce the association of E2F4 with p107 and p130. Taken together, these data suggest that the kinase activity of IE72 is required for E2F-dependent transcriptional activation and that this is likely to result from phosphorylation of specific members of the E2F and pocket protein families by IE72.

Metrics

9 Record Views
61 citations in Web of Science
61 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Biochemistry & Molecular Biology
Cell Biology
Logo image