Journal article
Identification of bone marrow stromal cells and expression of tyrosine hydroxylase gene in them
Sheng wu hua hsüeh yü sheng wu wu li hsüeh pao, v 35(6), pp 536-541
Jun 2003
PMID: 12796814
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The study is to establish the method of isolation and identification of bone marrow stromal cells and to investigate the ability of bone marrow stromal cells to accept and express TH gene. Cells were isolated by a density gradient (lymphocytes separation) and identified by BrdU labeling and fluorescence-activated cell sorting (FACS) technology using CD11b, CD45 and CD90 antibodies. TH and lacZ gene were transfected to rBMSCs with an adeno-associated virus vector. The results showed that most tightly adherent cells in the primary culture were fibroblast-like and formed foci of two to four cells. The cells in the foci remained dormant for 2 to 4 days and then began to multiply rapidly. After several passages, the adherent cells became more uniformly spindle-shaped in appearance. BrdU, indicating that BMSCs replicate actively, labeled about 74.9% of cultured cells. Data from FACS showed that about 75% of isolated cells were CD90(+)/CD45(-)/CD11b(-), which is the marker of bone marrow stromal cells. The efficiency of TH gene transfection was about 75%. BMSCs could readily be genetically engineered and could be useful delivery targets of gene therapy for Parkinson's disease.
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Details
- Title
- Identification of bone marrow stromal cells and expression of tyrosine hydroxylase gene in them
- Creators
- Ling-Ling Lu - Capital UniversityYu-Jin SuChun-Li ZhaoXi-Feng ZouShao-Ping HouQun-Yuan XuHui Yang
- Publication Details
- Sheng wu hua hsüeh yü sheng wu wu li hsüeh pao, v 35(6), pp 536-541
- Resource Type
- Journal article
- Language
- Chinese
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000183733400008
- Scopus ID
- 2-s2.0-0041885113
- Other Identifier
- 991020099955004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics