Journal article
Identification of differentially expressed genes in scarless wound healing utilizing polymerase chain reaction-suppression subtractive hybridization
Wound repair and regeneration, v 14(4), pp 413-420
Jul 2006
PMID: 16939568
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Wound healing in fetal skin is well known to proceed without scarring, whereas adult (postnatal) skin wound healing is accompanied by scar formation. To identify differentially expressed genes during fetal wound (FW) healing, we have used polymerase chain reaction-suppression subtractive hybridization. This technique allows for a comparative analysis across the entire transcriptome of FW vs. unwounded fetal control tissue, including even potentially novel sequences. Our subtractive hybridization protocol identified 15 clones that are overexpressed in healing FWs, and 20 clones that are underexpressed. These include genes with both known and unknown functions. We have confirmed the differential pattern of expression for four of these candidate genes: elongation factor 1 alpha, elongation initiation factor 4e, and two transcripts thus far known only as an expressed sequence tags. With this approach, we have also identified novel genes potentially involved in scarless wound healing.
Metrics
Details
- Title
- Identification of differentially expressed genes in scarless wound healing utilizing polymerase chain reaction-suppression subtractive hybridization
- Creators
- Sandeep Kathju - Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212-4772, USA. skathju@wpahs.orgLatha SatishCara RabikTerra RupertDuane OswaldSandra JohnsonFen Ze HuJ Christopher PostGarth D Ehrlich
- Publication Details
- Wound repair and regeneration, v 14(4), pp 413-420
- Publisher
- Wiley; United States
- Grant note
- DC04173 / NIDCD NIH HHS DC 05659 / NIDCD NIH HHS K08 DE014780 / NIDCR NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000239866100007
- Scopus ID
- 2-s2.0-33747620718
- Other Identifier
- 991014878002604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Dermatology
- Medicine, Research & Experimental
- Surgery