Journal article
Identification of differentially expressed genes in fibroblasts derived from patients with Dupuytren's Contracture
BMC medical genomics, v 1(1), pp 10-10
23 Apr 2008
PMID: 18433489
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Dupuytren's contracture (DC) is the most common inherited connective tissue disease of humans and is hypothesized to be associated with aberrant wound healing of the palmar fascia. Fibroblasts and myofibroblasts are believed to play an important role in the genesis of DC and the fibroproliferation and contraction that are hallmarks of this disease. This study compares the gene expression profiles of fibroblasts isolated from DC patients and controls in an attempt to identify key genes whose regulation might be significantly altered in fibroblasts found within the palmar fascia of Dupuytren's patients. Total RNA isolated from diseased palmar fascia (DC) and normal palmar fascia (obtained during carpal tunnel release; 6 samples per group) was subjected to quantitative analyses using two different microarray platforms (GE Code Linktrade mark and Illuminatrade mark) to identify and validate differentially expressed genes. The data obtained was analyzed using The Significance Analysis of Microarrays (SAM) software through which we identified 69 and 40 differentially regulated gene transcripts using the CodeLinktrade mark and Illuminatrade mark platforms, respectively. The CodeLinktrade mark platform identified 18 upregulated and 51 downregulated genes. Using the Illuminatrade mark platform, 40 genes were identified as downregulated, eleven of which were identified by both platforms. Quantitative RT-PCR confirmed the downregulation of three high-interest candidate genes which are all components of the extracellular matrix: proteoglycan 4 (PRG4), fibulin-1 (FBLN-1) transcript variant D, and type XV collagen alpha 1 chain. Overall, our study has identified a variety of candidate genes that may be involved in the pathophysiology of Dupuytren's contracture and may ultimately serve as attractive molecular targets for alternative therapies.
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Details
- Title
- Identification of differentially expressed genes in fibroblasts derived from patients with Dupuytren's Contracture
- Creators
- Latha Satish - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA. lsatish@wpahs.orgWilliam A LaFramboiseDavid B O'GormanSandra JohnsonBenjamin JantoBing Siang GanMark E BaratzFen Z HuJ Christopher PostGarth D EhrlichSandeep Kathju
- Publication Details
- BMC medical genomics, v 1(1), pp 10-10
- Publisher
- Springer BMC; England
- Grant note
- 84247 / Canadian Institutes of Health Research K08 DE014780 / NIDCR NIH HHS R01 DC004173 / NIDCD NIH HHS R01 DC005659 / NIDCD NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000272649800001
- Other Identifier
- 991014878104104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Genetics & Heredity