Journal article
Identification of the Site of Inhibition of Oncogenic ras-p21-Induced Signal Transduction by a Peptide from a ras Effector Domain
Journal of protein chemistry, v 18(8), pp 881-884
01 Nov 1999
PMID: 10839625
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have previously found that a peptide corresponding to residues 35-47 of the ras-p21 protein, from its switch 1 effector domain region, strongly inhibits oocyte maturation induced by oncogenic p21, but not by insulin-activated cellular wild-type p21. Another ras-p21 peptide corresponding to residues 96-110 that blocks ras-jun and jun kinase (JNK) interactions exhibits a similar pattern of inhibition. We have also found that c-raf strongly induces oocyte maturation and that dominant negative c-raf strongly blocks oncogenic p21-induced oocyte maturation. We now find that the p21 35-47, but not the 96-110, peptide completely blocks c-raf-induced maturation. This finding suggests that the 35-47 peptide blocks oncogenic ras at the level of raf; that activated normal and oncogenic ras-p21 have differing requirements for raf-dependent signaling; and that the two oncogenic-ras-selective inhibitory peptides, 35-47 and 96-110, act at two different critical downstream sites, the former at raf, the latter at JNK/jun, both of which are required for oncogenic ras-p21 signaling.[PUBLICATION ABSTRACT]
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Details
- Title
- Identification of the Site of Inhibition of Oncogenic ras-p21-Induced Signal Transduction by a Peptide from a ras Effector Domain
- Creators
- Lyndon Chie - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn, New York 11209, USA.James Chen - Computational Chemistry Division, Wyeth-Ayerst Corporation, Pearl RiverFred Friedman - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda,Denise Chung - Long Island UniversityShazia Amar - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn,Josef Michl - SUNY Downstate Health Sciences UniversityZ Yamaizumi - National Cancer Institute, Tokyo, JapanPaul Brandt-rauf - Royal College of PhysiciansMatthew Pincus - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn
- Publication Details
- Journal of protein chemistry, v 18(8), pp 881-884
- Publisher
- Springer Nature B.V
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000087092800009
- Scopus ID
- 2-s2.0-53149153603
- Other Identifier
- 991019323776304721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology