Journal article
Identifying the Membrane Proteome of HIV-1 Latently Infected Cells
The Journal of biological chemistry, v 282(11), pp 8207-8218
16 Mar 2007
PMID: 17237230
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Profiling integral plasma membrane proteins is of particular importance for the identification of new biomarkers for diagnosis and for drug development. We report in this study the identification of surface markers by performing comparative proteomics of established human immunodeficiency virus-1 (HIV-1) latent cell models and parental cell lines. To this end we isolated integral membrane proteins using a biotin-directed affinity purification method. Isolated proteins were separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) after in gel digestion. Seventeen different proteins were found to vary on the surface of T-cells due to HIV-1 infection. Of these proteins, 47% were integral membrane proteins, and 18% were membrane-associated. Through the use of complementary techniques such as Western blotting and fluorescent staining, we confirmed the differential expression of some of the proteins identified by MALDI-TOF including Bruton's tyrosine kinase and X-linked inhibitor of apoptosis. Finally, using phosphatidylinositol 3-kinase inhibitors and flavopiridol to inhibit Bruton's tyrosine kinase localization at the membrane and X-linked inhibitor of apoptosis protein expression, respectively, we showed that HIV-1 latently infected cells are more sensitive to these drugs than uninfected cells. This suggests that HIV-1 latently infected cells may be targeted with drugs that alter several pathways that are essential for the establishment and maintenance of latency.
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Details
- Title
- Identifying the Membrane Proteome of HIV-1 Latently Infected Cells
- Creators
- Reem Berro - George Washington UniversityCynthia de la Fuente - George Washington UniversityZachary Klase - George Washington UniversityKylene Kehn - George Washington UniversityLida Parvin - George Washington UniversityAnne Pumfery - George Washington UniversityEmmanuel Agbottah - George Washington UniversityAkos Vertes - George Washington UniversitySergei Nekhai - Howard UniversityFatah Kashanchi - George Washington University
- Publication Details
- The Journal of biological chemistry, v 282(11), pp 8207-8218
- Publisher
- Elsevier
- Number of pages
- 12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000245081000053
- Scopus ID
- 2-s2.0-34247255381
- Other Identifier
- 991021902599604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology