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IgE-Dependent Expression of Interleukin-5 mRNA and Protein in Human Lung: Modulation by Dexamethasone
Journal article

IgE-Dependent Expression of Interleukin-5 mRNA and Protein in Human Lung: Modulation by Dexamethasone

Mark C Glaum, Jonathan S Jaffe, David H Gillespie, Donald G Raible, Thomas J Post, Yihe Wang, Edward Dimitry and Edward S Schulman
Clinical immunology and immunopathology, v 75(2), pp 171-178
May 1995
DOI: https://doi.org/10.1006/clin.1995.1068
PMID: 7704976
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

mRNA and protein expression of the Th2 cytokines IL-4 and IL-5 from human lung were examined during the first 4 hr following IgE-mediated triggering, a time representative of the evolving late-phase reaction (LPR). Lung explants were incubated for 16 hr at 37°C in culture media alone or with added dexamethasone (10-6M), washed, and then challenged with buffer or anti-IgE (3 μg/ml). Using RNase protection assays, in 16/16 individual lungs IL-5 mRNA expression was observed at 4 hr following anti-IgE and at no points following buffer challenge. Fragments released 1129 ± 499 ng of IL-5/g wet wt over a 24-hr period (mean ± SEM, n = 5). Neither IL-4 transcripts nor protein were detected in any anti-IgE challenges. Both the IgE-mediated IL-5 mRNA and protein responses were below the limits of detection following dexamethasone preincubation, suggesting a mechanism for the potent inhibitory effects of these agents observed in the LPR.

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Web of Science research areas
Immunology
Pathology
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