Journal article
Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis
The Journal of nuclear medicine (1978), v 43(10), pp 1359-1365
01 Oct 2002
PMID: 12368374
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
UNLABELLEDRheumatoid arthritis is associated with chronic synovial inflammation due to the abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. The autoreactive cells cause an inflammatory proapoptotic response to self-antigens resulting in eventual bone, cartilage, and soft-tissue loss and destruction. The goal of our study was to determine the timing and intensity of apoptosis in joints using 99mTc-labeled annexin V, an in vivo marker of apoptosis, in a murine model of immune arthritis.METHODSWe used 99mTc-annexin V and autoradiography to study the extent and severity of apoptosis in the front and rear paws of DBA/1 mice with type II collagen-induced rheumatoid arthritis.RESULTSCompared with control values (n = 10), there was a significant (P < 0.002) nearly 3-fold increase in uptake of 99mTc-annexin V in the front foot pads, rear toes, rear foot pads, and heels at the time of maximal extremity swelling as determined by serial caliper measurements at 4 wk after inoculation with type II bovine collagen (n = 9). The front toes had a 5- to 6-fold increase in uptake compared with control values (P < 0.001). Histologic analysis revealed only scattered rare lymphocytes in the periarticular soft tissues, without joint destruction. Dual autoradiography with 125I-bovine serum albumin as a control showed that 99mTc-annexin V localization was specific. Treatment with methylprednisolone for 1 wk (n = 8) at 4 wk after immunization with type II collagen decreased 99mTc-annexin V uptake by 3- to 6-fold compared with control values (P < 0.002).CONCLUSION99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction.
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Details
- Title
- Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis
- Creators
- Anneke M PostPeter D KatsikisJonathan F TaitSharon M GeaghanH William StraussFrancis G Blankenberg
- Publication Details
- The Journal of nuclear medicine (1978), v 43(10), pp 1359-1365
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000178393900018
- Scopus ID
- 2-s2.0-0036787658
- Other Identifier
- 991019348755304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Radiology, Nuclear Medicine & Medical Imaging