Journal article
Immortalization of a cell line showing some characteristics of the oligodendrocyte phenotype
Neuroscience letters, v 159(1)
1993
PMID: 8264960
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have used the polyoma middle T oncogene to immortalize cells from rat embryo encephalon. Immunostaining experiments with monoclonal antibodies demonstrated that the cells of one of the obtained lines, named CEINGE CL3, are stained by anti-vimentin and anti-S100 antibodies, are not stained by anti-neurofilaments (NF) or anti-glial fibrillary acidic protein (GFAP) antibodies. Only a subset of the CEINGE CL3 cells (20–30%) is stained by an anti-galactocerebroside antibody. Northern blot analysis demonstrated that these cells express low levels of proteolipid protein mRNA, whereas polymerase chain reaction (PCR) amplification failled to evidentiate the presence of both NF and GFAP mRNAs. Either retinoic acid or forskolin treatments or a combination of them are able to induce morphological changes that are accompanied by a complete growth arrest.
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Details
- Title
- Immortalization of a cell line showing some characteristics of the oligodendrocyte phenotype
- Creators
- Tommaso Russo - University of Naples Federico IIAnna Rita Mogavero - Ceinge Biotecnologie Avanzate (Italy)Rosario Ammendola - Ceinge Biotecnologie Avanzate (Italy)Maria Mesuraca - Ceinge Biotecnologie Avanzate (Italy)Francesca Fiore - Ceinge Biotecnologie Avanzate (Italy)Alessandro Fatatis - Ceinge Biotecnologie Avanzate (Italy)Gaetano Salvatore - Ceinge Biotecnologie Avanzate (Italy)Filiberto Cimino - Ceinge Biotecnologie Avanzate (Italy)
- Publication Details
- Neuroscience letters, v 159(1)
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Pharmacology and Physiology; Drexel University
- Web of Science ID
- WOS:A1993LY62100041
- Scopus ID
- 2-s2.0-0027270969
- Other Identifier
- 991020100201404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences