Journal article
Immunoassays of the Metalloproteinase (MMP-2) and Tissue Inhibitor of Metalloproteinase (TIMP1 and 2) Levels in Noninvasive and Metastatic PC-3 Clones: Effects of Taxol
Oncology research, v 6(4-5)
01 Jan 1994
PMID: 7841542
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Abstract We have selected and subcloned bone metastatic (PC-3 ML) and noninvasive, nonmetastatic (3 × N.I.) lines from human prostatic PC-3 parent cells. In this paper, we have compared relative levels of MMP- 2 and TIMP-1 and 2 in PC-3 tumors grown in severe combined
immunodeficient or SCID mice. Dot blots with polyclonal antibodies specific for MMP-2, TIMP-1 and TIMP-2 revealed that the MMP-2 levels were high in subcutaneously grown PC-3 ML clones but low in 3 × N.I. clones at days 20 and 40. The TIMP-1 levels were inversely proportional to MMP-2
in the two types of clones, respectively. The TIMP-2 levels were similar in both clones at days 20 and 40. Gelatin zymograms confirmed that PC-3 ML tumors contained MMP-2 (and not MMP-9) subcutaneously or in bone metastases in SCID mice. Slot blots of PC-3 ML bone tumors comparing MMP-2 and
TIMP-1 levels showed at days 10, 15, 17, 19, 21, and 25 that the ratio of MMP-2 to TIMP-1 increased, especially at about day 21, when extensive secondary metastases to the peritoneal cavity occurred. The levels of TIMP-2 remained constant. Quantitative ELIS As confirmed the blotting data and
showed that taxol blocked MMP-2 but not TIMP-1 production in these advanced tumors. We conclude that highly metastatic PC-3 ML variants contained relatively high levels of MMP-2 and low amounts of TIMP-1.
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Details
- Title
- Immunoassays of the Metalloproteinase (MMP-2) and Tissue Inhibitor of Metalloproteinase (TIMP1 and 2) Levels in Noninvasive and Metastatic PC-3 Clones: Effects of Taxol
- Creators
- Mark E StearnsMin Wang
- Publication Details
- Oncology research, v 6(4-5)
- Publisher
- Cognizant Communication Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Decision Sciences (and Management Information Systems)
- Web of Science ID
- WOS:A1994PK79800004
- Scopus ID
- 2-s2.0-0027967237
- Other Identifier
- 991019183984304721
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- Oncology