Journal article
Immunological idenTIFication of human platelet mitochondrial DNA topoisomerase I
Biochimica et biophysica acta, Protein structure and molecular enzymology, v 1164(1), pp 101-107
1993
PMID: 8390858
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Anuclear human blood platelets have been used to study mitochondrial topoisomerase activity in the absence of nuclear contamination. Previous work utilizing this novel system demonstrated that platelet mitochondria contain type-I topoisomerase (Kosovsky, M.J. and Soslau, G. (1991) Biochim. Biophys. Acta 1078, 56–62). The present work has demonstrated that mitochondrial topoisomerase activity was inhibited by the specific topoisomerase-I inhibitor, topotecan, yet was not affected by a specific topoisomerase-II inhibitor, VM-26. These results confirm that platelet mitochondria contain topoisomerase I, yet do not contain a detectable level of topoisomerase-II activity. It has been demonstrated for the first time that antibodies directed against nuclear topo I cross-react with mitochondrial topo I. Furthermore, immunoblot analysis of platelet mitochondrial proteins, in conjunction with renaturation studies, has led to the identification of a catalytically active 60-kDa form of mitochondrial topoisomerase I.
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Details
- Title
- Immunological idenTIFication of human platelet mitochondrial DNA topoisomerase I
- Creators
- Marshall J Kosovsky - Department of Biological Chemistry, Hahnemann University, Philadelphia, PA USAGerald Soslau - Department of Biological Chemistry, Hahnemann University, Philadelphia, PA USA
- Publication Details
- Biochimica et biophysica acta, Protein structure and molecular enzymology, v 1164(1), pp 101-107
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1993LK41400014
- Scopus ID
- 2-s2.0-0027238738
- Other Identifier
- 991014878368104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics