Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial

Jacques Ravel; Sheri Simmons; Eleni Greenwood Jaswa; Sara Gottfried; Miriam Greene; Susan Kellogg-Spadt; Dirk Gevers; Diane M Harper

doi:10.1038/s41522-025-00788-6

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Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial

Journal article

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Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial

Jacques Ravel, Sheri Simmons, Eleni Greenwood Jaswa, Sara Gottfried, Miriam Greene, Susan Kellogg-Spadt, Dirk Gevers and Diane M Harper

NPJ biofilms and microbiomes, v 11(1), 158

09 Aug 2025

DOI: https://doi.org/10.1038/s41522-025-00788-6

PMID: 40783570

Featured in Collection : UN Sustainable Development Goals @ Drexel

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Abstract

Adult

Candida - isolation & purification

Female

Gardnerella vaginalis - isolation & purification

Humans

Lactobacillus crispatus - physiology

Microbiota

Synbiotics - administration & dosage

Vagina - microbiology

Young Adult

A clinical trial of a multi-strain vaginal synbiotic (NCT05659745, registered 12/19/2022 at clinicaltrials.gov) led to an optimal vaginal microbiome dominated by L. crispatus (CST I). The synbiotic led to a significant increase in L. crispatus compared to placebo (p < 0.05), and conversion to CST I was significantly higher with the vaginal synbiotic than with placebo (90 vs 11%; p < 0.002). Mechanistically, the synbiotic reduced Gardnerella vaginalis and Candida, clinically important microbes.

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Title: Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial
Creators: Jacques Ravel - Center for Advanced Microbiome Research and Innovation, Institute for Genome Sciences and Department of Microbiology & Immunology, University of Maryland, School of Medicine, Baltimore, MD, USA
Sheri Simmons - Seed Health, Venice, CA, USA
Eleni Greenwood Jaswa - Department of Obstetrics, Gynecology & Reproductive Sciences, University of California, San Francisco, CA, USA
Sara Gottfried - Thomas Jefferson University
Miriam Greene - Department of Obstetrics & Gynecology, NYU Grossman School of Medicine, New York, NY, USA
Susan Kellogg-Spadt - Drexel University, Obstetrics and Gynecology
Dirk Gevers (Corresponding Author) - Seed Health, Venice, CA, USA. dirk@seed.com
Diane M Harper - University of Michigan
Publication Details: NPJ biofilms and microbiomes, v 11(1), 158
Publisher: Nature Publishing Group
Resource Type: Journal article
Language: English
Academic Unit: Obstetrics and Gynecology
Web of Science ID: WOS:001547187200003
Scopus ID: 2-s2.0-105012962545
Other Identifier: 991022073153004721

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Collaboration types: Domestic collaboration
Web of Science research areas: Biotechnology & Applied Microbiology; Microbiology

Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial

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Abstract

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UN Sustainable Development Goals (SDGs)

InCites Highlights

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