Journal article
Impact of cryopreservation on elastomuscular artery mechanics
Journal of the mechanical behavior of biomedical materials, v 154, pp 106503-106503
01 Jun 2024
PMID: 38522154
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Low temperatures slow or halt undesired biological and chemical processes, protecting cells, tissues, and organs during storage. Cryopreservation techniques, including controlled media exchange and regulated freezing conditions, aim to mitigate the physical consequences of freezing. Dimethyl sulfoxide (DMSO), for example, is a penetrating cryoprotecting agent (CPA) that minimizes ice crystal growth by replacing intracellular water, while polyvinyl alcohol (PVA) is a nonpenetrating CPA that prevents recrystallization during thawing. Since proteins and ground substance dominate the passive properties of soft biological tissues, we studied how different freezing rates, storage temperatures, storage durations, and the presence of cryoprotecting agents (5% [v/v] DMSO + 1 mg/mL PVA) impact the histomechanical properties of the internal thoracic artery (ITA), a clinically relevant blood vessel with both elastic and muscular characteristics. Remarkably, biaxial mechanical analyses failed to reveal significant differences among the ten groups tested, suggesting that mechanical properties are virtually independent of the cryopreservation technique. Scanning electron microscopy revealed minor CPA-independent delamination in rapidly frozen samples, while cryoprotected ITAs had better post-thaw viability than their unprotected counterparts using methyl thiazole-tetrazolium (MTT) metabolic assays, especially when frozen at a controlled rate. These results can be used to inform ongoing and future studies in vascular engineering, physiology, and mechanics.
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Details
- Title
- Impact of cryopreservation on elastomuscular artery mechanics
- Creators
- Colton J. Kostelnik - University of South CarolinaKiersten J. Crouse - University of South CarolinaJackson D. Goldsmith - University of South CarolinaJohn F. Eberth - Drexel University
- Publication Details
- Journal of the mechanical behavior of biomedical materials, v 154, pp 106503-106503
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- Koerner Family Foundation Doctoral Student Fellowship OIA-1655740; CMMI 1760906 / National Science Foundation (EPSCoR); National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001219595600001
- Scopus ID
- 2-s2.0-85188648935
- Other Identifier
- 991021902501104721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials