Journal article
Impact of soil clay minerals on growth, biofilm formation, and virulence gene expression of Escherichia coli O157:H7
Environmental pollution (1987), v 243(Pt B), pp 953-960
01 Dec 2018
PMID: 30245456
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Soil, composed mainly of minerals, plays a central role in the circulation of microbial pathogens in the environment. Herein, the growth, biofilm formation, and virulence gene expression of the pathogenic bacteria Escherichia coli O157:H7 were monitored following exposure to montmorillonite, kaolinite, and goethite, three common soil minerals in the clay size fraction. E. coli O157:H7 growth was notably promoted (P < 0.05), while biofilm formation was inhibited in the presence of montmorillonite (P < 0.05), which is attributed to the suppression of colanic acid (CA) production and an increase in bacterial motility. Kaolinite not only promoted bacterial growth (P < 0.05), but also contributed to biofilm formation upon stimulating CA production. Upon exposure to goethite, notably slower bacterial growth and higher biomass of biofilm were observed as compared to the control (P < 0.05). Goethite stimulated the synthesis of CA to encase cells in a protective biofilm in response to the tight association between bacteria and goethite, which could cause bacterial death. Additionally, the transcription of virulence factors (stxA-1 and stxA-2) was significantly decreased in goethite or kaolinite system (P < 0.05). These findings indicate that minerals play important roles in the physiological state of bacteria and ultimately govern the fate of this pathogen in soils. (C) 2018 Elsevier Ltd. All rights reserved.
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Details
- Title
- Impact of soil clay minerals on growth, biofilm formation, and virulence gene expression of Escherichia coli O157:H7
- Creators
- Peng Cai - Huazhong Agricultural UniversityXing Liu - Huazhong Agricultural UniversityDandan Ji - Huazhong Agricultural UniversityShanshan Yang - Huazhong Agricultural UniversitySharon L. Walker - University of California, RiversideYichao Wu - Huazhong Agricultural UniversityChunhui Gao - Huazhong Agricultural UniversityQiaoyun Huang - Huazhong Agricultural University
- Publication Details
- Environmental pollution (1987), v 243(Pt B), pp 953-960
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- 41522106 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2662015PY081 / Fundamental Research Funds for the Central Universities 2016YFD0800206 / National Key Research Program of China
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000449892700018
- Scopus ID
- 2-s2.0-85054687830
- Other Identifier
- 991021229898104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Environmental Sciences