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Journal article
Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2
The Journal of biological chemistry, v 297(4), 101151
Oct 2021
PMID: 34478710
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The seasonal nature of outbreaks of respiratory viral infections with increased transmission during low temperatures has been well established. Accordingly, temperature has been suggested to play a role on the viability and transmissibility of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. The receptor-binding domain (RBD) of the Spike glycoprotein is known to bind to its host receptor angiotensin-converting enzyme 2 (ACE2) to initiate viral fusion. Using biochemical, biophysical, and functional assays to dissect the effect of temperature on the receptor–Spike interaction, we observed a significant and stepwise increase in RBD-ACE2 affinity at low temperatures, resulting in slower dissociation kinetics. This translated into enhanced interaction of the full Spike glycoprotein with the ACE2 receptor and higher viral attachment at low temperatures. Interestingly, the RBD N501Y mutation, present in emerging variants of concern (VOCs) that are fueling the pandemic worldwide (including the B.1.1.7 (α) lineage), bypassed this requirement. This data suggests that the acquisition of N501Y reflects an adaptation to warmer climates, a hypothesis that remains to be tested.
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Details
- Title
- Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2
- Creators
- Jérémie Prévost - University of MontrealJonathan Richard - University of MontrealRomain Gasser - University of MontrealShilei Ding - Centre Hospitalier de l’Université de MontréalClément Fage - Université LavalSai Priya Anand - McGill UniversityDamien Adam - University of MontrealNatasha Gupta Vergara - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USAAlexandra Tauzin - University of MontrealMehdi Benlarbi - Centre Hospitalier de l’Université de MontréalShang Yu Gong - McGill UniversityGuillaume Goyette - Centre Hospitalier de l’Université de MontréalAnik Privé - Centre Hospitalier de l’Université de MontréalSandrine Moreira - Institut National de Santé Publique du QuébecHugues Charest - Institut National de Santé Publique du QuébecMichel Roger - University of MontrealWalther Mothes - Yale UniversityMarzena Pazgier - Uniformed Services University of the Health SciencesEmmanuelle Brochiero - University of MontrealGuy Boivin - Université LavalCameron F. Abrams - Drexel UniversityArne Schön - Johns Hopkins UniversityAndrés Finzi - University of Montreal
- Publication Details
- The Journal of biological chemistry, v 297(4), 101151
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000713011000001
- Scopus ID
- 2-s2.0-85114907952
- Other Identifier
- 991019169804404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology