Journal article
Impact of viral activators and epigenetic regulators on HIV-1 LTRs containing naturally occurring single nucleotide polymorphisms
BioMed research international, v 2015, 320642
2015
PMID: 25629043
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Following human immunodeficiency virus type 1 (HIV-1) integration into host cell DNA, the viral promoter can become transcriptionally silent in the absence of appropriate signals and factors. HIV-1 gene expression is dependent on regulatory elements contained within the long terminal repeat (LTR) that drive the synthesis of viral RNAs and proteins through interaction with multiple host and viral factors. Previous studies identified single nucleotide polymorphisms (SNPs) within CCAAT/enhancer binding protein (C/EBP) site I and Sp site III (3T, C-to-T change at position 3, and 5T, C-to-T change at position 5 of the binding site, respectively, when compared to the consensus B sequence) that are low affinity binding sites and correlate with more advanced stages of HIV-1 disease. Stably transfected cell lines containing the wild type, 3T, 5T, and 3T5T LTRs were developed utilizing bone marrow progenitor, T, and monocytic cell lines to explore the LTR phenotypes associated with these genotypic changes from an integrated chromatin-based microenvironment. Results suggest that in nonexpressing cell clones LTR-driven gene expression occurs in a SNP-specific manner in response to LTR activation or treatment with trichostatin A treatment, indicating a possible cell type and SNP-specific mechanism behind the epigenetic control of LTR activation.
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Details
- Title
- Impact of viral activators and epigenetic regulators on HIV-1 LTRs containing naturally occurring single nucleotide polymorphisms
- Creators
- Sonia Shah - Department of Microbiology and Immunology, Drexel University College of Medicine School of Medicine, 245 N. 15th Street, MS1013A, Rm 18301, Philadelphia, PA 19102, USA ; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine School of Medicine, Philadelphia, PA 19102, USAVanessa Pirrone - Department of Microbiology and Immunology, Drexel University College of Medicine School of Medicine, 245 N. 15th Street, MS1013A, Rm 18301, Philadelphia, PA 19102, USA ; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine School of Medicine, Philadelphia, PA 19102, USAAikaterini Alexaki - Department of Microbiology and Immunology, Drexel University College of Medicine School of Medicine, 245 N. 15th Street, MS1013A, Rm 18301, Philadelphia, PA 19102, USA ; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine School of Medicine, Philadelphia, PA 19102, USAMichael R Nonnemacher - Department of Microbiology and Immunology, Drexel University College of Medicine School of Medicine, 245 N. 15th Street, MS1013A, Rm 18301, Philadelphia, PA 19102, USA ; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine School of Medicine, Philadelphia, PA 19102, USABrian Wigdahl - Department of Microbiology and Immunology, Drexel University College of Medicine School of Medicine, 245 N. 15th Street, MS1013A, Rm 18301, Philadelphia, PA 19102, USA ; Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine School of Medicine, Philadelphia, PA 19102, USA
- Publication Details
- BioMed research international, v 2015, 320642
- Publisher
- Wiley; United States
- Grant note
- DA19807 / NIDA NIH HHS R01 DA019807 / NIDA NIH HHS NS46263 / NINDS NIH HHS P30 MH-092177 / NIMH NIH HHS R01 NS032092 / NINDS NIH HHS 5T32MH079785 / NIMH NIH HHS NS32092 / NINDS NIH HHS R01 NS046263 / NINDS NIH HHS P30 MH092177 / NIMH NIH HHS T32 MH079785 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000348291600001
- Scopus ID
- 2-s2.0-84921419570
- Other Identifier
- 991014878238804721
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- Web of Science research areas
- Biotechnology & Applied Microbiology
- Medicine, Research & Experimental