Journal article
Improved NYVAC-Based Vaccine Vectors
PloS one, v 6(11), pp e25674-e25674
09 Nov 2011
PMCID: PMC3212513
PMID: 22096477
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
While as yet there is no vaccine against HIV/AIDS, the results of the phase III Thai trial (RV144) have been encouraging and suggest that further improvements of the prime/boost vaccine combination of a poxvirus and protein are needed. With this aim, in this investigation we have generated derivatives of the candidate vaccinia virus vaccine vector NYVAC with potentially improved functions. This has been achieved by the re-incorporation into the virus genome of two host range genes,
K1L
and
C7L
, in conjunction with the removal of the immunomodulatory viral molecule B19, an antagonist of type I interferon action. These novel virus vectors, referred to as NYVAC-C-KC and NYVAC-C-KC-ΔB19R, have acquired relevant biological characteristics, giving higher levels of antigen expression in infected cells, replication-competency in human keratinocytes and dermal fibroblasts, activation of selective host cell signal transduction pathways, and limited virus spread in tissues. Importantly, these replication-competent viruses have been demonstrated to maintain a highly attenuated phenotype.
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Details
- Title
- Improved NYVAC-Based Vaccine Vectors
- Creators
- Karen V. Kibler - University of ChicagoCarmen E. Gomez - University of ChicagoBeatriz Perdiguero - University of ChicagoShukmei Wong - University of ChicagoTrung Huynh - University of ChicagoSusan Holechek - University of ChicagoWilliam Arndt - University of ChicagoVictoria Jimenez - University of ChicagoRuben Gonzalez-Sanz - University of ChicagoKaren Denzler - University of ChicagoElias K. Haddad - University of ChicagoRalf Wagner - University of RegensburgRafick P. Sékaly - Université de MontréalJames Tartaglia - University of ChicagoGiuseppe Pantaleo - University Hospital of LausanneBertram L. Jacobs - University of ChicagoMariano Esteban - University of Chicago
- Publication Details
- PloS one, v 6(11), pp e25674-e25674
- Publisher
- Public Library of Science
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Infectious Diseases (and HIV Medicine); Drexel University
- Web of Science ID
- WOS:000297350800002
- Scopus ID
- 2-s2.0-80655128531
- Other Identifier
- 991020099634904721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology