Journal article
Improved PSD and CID on a MALDI TOFMS
Journal of the American Society for Mass Spectrometry, v 15(4), pp 523-535
2004
PMID: 15047057
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The influence of several instrument-operating parameters on the product-ion resolution and mass accuracy in matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) post-source decay (PSD) and collision-induced dissociation (CID) experiments is reported. Voltages commonly applied to the reflectron for PSD and CID experiments were found to be non-ideal; optimization of these voltages resulted in better resolution across each segment of the measured PSD spectrum. Mass resolution, calculated as M/ΔM (FWHM) for the product-ion peaks, was as high as 2500. Additionally, precursor-ion selection and segment mass range setup were each found to have dramatic influences on product-ion mass accuracy. An understanding of the influence of these variables aided in the interpretation of (a-NH
3) and (b − NH
3) ions observed in the PSD/CID spectra of a number of peptides. In addition, product ions resulting from coincidence peaks in the precursor-ion selection window were found to be a general problem. With the improvements to resolution and optimization of these mass accuracy variables, the mass accuracy of product ions from MALDI TOF PSD and CID experiments was tested with several reference materials, including the peptides Substance P, bradykinin, angiotensin I, and angiotensin II and the synthetic polymers poly(methyl methacrylate) and polystyrene. The absolute error (Da) for each test material was, on average, below 0.1 Da, demonstrating a significant improvement in mass accuracy using the improved operational parameters and an extension of the use of poly(ethylene glycol) (PEG) as a mass calibrant for the PSD/CID spectra.
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Details
- Title
- Improved PSD and CID on a MALDI TOFMS
- Creators
- Andrew J Hoteling - Imaging Materials and Media, R&D, Eastman Kodak Company, Rochester, New York, USAKevin G Owens - Department of Chemistry, Drexel University, Philadelphia, Pennsylvania, USA
- Publication Details
- Journal of the American Society for Mass Spectrometry, v 15(4), pp 523-535
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000220506000009
- Scopus ID
- 2-s2.0-1642421718
- Other Identifier
- 991014877990304721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Physical
- Spectroscopy