Journal article
Improved protein detection on an AC electrokinetic quartz crystal microbalance (EKQCM)
Biosensors & bioelectronics, v 26(8), pp 3391-3397
2011
PMID: 21353520
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microscale electrodes supplied with an AC field can generate rotational fluid patterns known as AC electroosmosis. In the present study, this effect was used to improve antibody binding on a biosensor surface. Antibodies, like many other large, slow moving biomolecules, tend to suffer from transport limitations during a reaction with a surface-bound receptor. Stirring such reactions with AC electroosmosis can alleviate this transport limitation by bringing fresh reagent to the surface. For the first time, the use of this phenomenon was used to improve the capture of protein on a sensor. Directly adsorbed antibodies were bound to the surface of specially modified quartz crystal microbalances, known as electrokinetic QCMs (EKQCMs) and the signal was enhanced by about 5.6 times. Modification of the QCM resulted in little reduction of quality factor (from ∼5.3k to ∼4.6k) and an increased sensitivity to viscosity changes (151%). Full immunoassays performed on electrodes fabricated on glass surfaces were used to ensure antibody function was not significantly degraded by the enhancement technique.
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Details
- Title
- Improved protein detection on an AC electrokinetic quartz crystal microbalance (EKQCM)
- Creators
- Robert Hart - School of Biomedical Engineering, Science & Health Systems, Drexel University, United StatesErten Ergezen - School of Biomedical Engineering, Science & Health Systems, Drexel University, United StatesRyszard Lec - School of Biomedical Engineering, Science & Health Systems, Drexel University, United StatesHongseok “Moses” Noh - Department of Mechanical Engineering & Mechanics, Drexel University, 3141 Chestnut St., Rm. 2-115, Philadelphia, PA 19104, United States
- Publication Details
- Biosensors & bioelectronics, v 26(8), pp 3391-3397
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000289863900001
- Scopus ID
- 2-s2.0-79952814353
- Other Identifier
- 991014878225404721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology