Journal article
In Situ Detection of Protein Complexes and Modifications by Chemical Ligation Proximity Assay
Bioconjugate chemistry, v 27(7), pp 1690-1696
20 Jul 2016
PMID: 27248443
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Protein function is often regulated by protein-protein interactions and post-translational modifications. Detection of these important biological phenomena in fixed biological samples could serve as an invaluable tool in biomedical research, drug development, as well as clinical cancer diagnostics and prognostics. We report here a novel methodology which utilizes unique antibody bioconjugates capable of forming proximity induced chemical ligation to enable in situ detection of proximal targets in fixed biological samples. Using this new methodology, we demonstrate in situ visualization of various protein heterodimers/complexes and post-translational modifications such as phosphorylation and ubiquitination. This new method offers high specificity, sensitivity, flexibility, and ease of use. In addition, the assay preserves critical contextual and heterogeneity information on biomarkers in clinically relevant samples.
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Details
- Title
- In Situ Detection of Protein Complexes and Modifications by Chemical Ligation Proximity Assay
- Creators
- Rui Hong - Ventana Medical SystemsEsteban Roberts - Ventana Medical SystemsChristopher Bieniarz - Ventana Medical SystemsChristopher B Rodell - School of Biomedical Engineering, Science, and Health Systems (1997-)
- Publication Details
- Bioconjugate chemistry, v 27(7), pp 1690-1696
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000380298900018
- Scopus ID
- 2-s2.0-84979590881
- Other Identifier
- 991019320608904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- Chemistry, Organic