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Journal article
In Vitro Anti-hepatitis B Virus Activity of 2′,3′-Dideoxyguanosine
Virologica Sinica, v 33(6), pp 538-544
01 Dec 2018
PMID: 30421112
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
2′,3′-dideoxyguanosine (DoG) has been demonstrated to inhibit duck hepatitis B virus (DHBV) replication
in vivo
in a duck model of HBV infection. In the current study, the
in vitro
antiviral effects of DoG on human and animal hepadnaviruses were investigated. Our results showed that DoG effectively inhibited HBV, DHBV, and woodchuck hepatitis virus (WHV) replication in hepatocyte-derived cells in a dose-dependent manner, with 50% effective concentrations (EC
50
) of 0.3 ± 0.05, 6.82 ± 0.25, and 23.0 ± 1.5 μmol/L, respectively. Similar to other hepadnaviral DNA polymerase inhibitors, DoG did not alter the levels of intracellular viral RNA but induced the accumulation of a less-than-full-length viral RNA species, which was recently demonstrated to be generated by RNase H cleavage of pgRNA. Furthermore, using a transient transfection assay, DoG showed similar antiviral activity against HBV wild-type, 3TC-resistant rtA181V, and adefovir-resistant rtN236T mutants. Our results suggest that DoG has potential as a nucleoside analogue drug with anti-HBV activity.
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Details
- Title
- In Vitro Anti-hepatitis B Virus Activity of 2′,3′-Dideoxyguanosine
- Creators
- Pinghu Zhang - Yangzhou UniversityShuo Zhai - Yangzhou UniversityJinhong Chang - Baruch S. Blumberg InstituteJu-Tao Guo - Baruch S. Blumberg Institute
- Publication Details
- Virologica Sinica, v 33(6), pp 538-544
- Publisher
- Springer Singapore
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000455958600010
- Scopus ID
- 2-s2.0-85056353550
- Other Identifier
- 991020547614204721
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- Collaboration types
- International collaboration
- Web of Science research areas
- Virology