Journal article
In Vitro Cell Shearing Device to Investigate the Dynamic Response of Cells in a Controlled Hydrodynamic Environment
Annals of biomedical engineering, v 28(4), pp 363-372
Apr 2000
PMID: 10870893
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mechanical stresses and strains play important roles in the normal growth and development of biological tissues, yet the cellular mechanisms of mechanotransduction have not been identified. A variety of in vitro systems for applying mechanical loads to cell populations have been developed to gain insight into these mechanisms. However, limitations in the ability to control precisely relevant aspects of the mechanical stimuli have obscured the physical relationships between mechanical loading and the biochemical signals that mediate the cellular response. We present a novel in vitro cell shearing device based on the principles of a cone and plate viscometer that utilizes microstepper motor technology to control independently the dynamic and steady components of a hydrodynamic shear-stress environment. Physical measurements of the cone velocity demonstrated faithful reproduction of user-defined input wave forms. Computational modeling of the fluid environment for the unsteady startup confirmed small inertial contributions and negligible secondary flows. Finally, we present experimental results demonstrating the onset rate dependence of functional and structural responses of endothelial cell cultures to dynamically applied shear stress. The controlled cell shearing device is a novel tool for elucidating mechanisms by which mechanical forces give rise to the biological signals that modulate cellular morphology and metabolism. © 2000 Biomedical Engineering Society.
PAC00: 8780Rb, 8717-d
Metrics
Details
- Title
- In Vitro Cell Shearing Device to Investigate the Dynamic Response of Cells in a Controlled Hydrodynamic Environment
- Creators
- Brett Blackman - Department of Bioengineering University of Pennsylvania Philadelphia PAKenneth Barbee - School of Biomedical Engineering, Science, and Health Systems Drexel University Philadelphia PALawrence Thibault - School of Biomedical Engineering, Science, and Health Systems Drexel University Philadelphia PA
- Publication Details
- Annals of biomedical engineering, v 28(4), pp 363-372
- Publisher
- Kluwer Academic Publishers-Plenum Publishers; New York
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000087518500002
- Scopus ID
- 2-s2.0-0033623705
- Other Identifier
- 991014878062004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical