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Journal article
In Vitro and In Vivo Evaluation of Ultrasound-Triggered Release From Novel Spinal Device
Journal of ultrasound in medicine
30 May 2023
PMID: 37249416
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bacterial infection following spinal fusion is a major clinical concern with up to 20% incidence. An ultrasound-triggered bulk-release system to combat postsurgical bacterial survival was designed and evaluated.
Polylactic acid (PLA) clips were loaded with vancomycin (VAN) and microbubbles (Sonazoid, GE HealthCare) in vitro. Stability was determined over 14 days. VAN-loaded clips were submerged in water and insonated using a Logiq E10 scanner (GE HealthCare) with a curvilinear C6 probe. Doppler-induced VAN release was quantified using spectrophotometry. For in vivo testing, clips were loaded with methylene blue (MeB) solution and Sonazoid. These clips were implanted into a rabbit along the spine at L2 and L5, as well as a pig at L1 and L3, then insonated in Doppler mode using the C6 probe.
Sonazoid microbubbles were better preserved when incubated in VAN compared with distilled water at 4°C, 25°C, and 37°C incubation temperatures (P = .0131). Contrast enhancement was observed from both solutions when incubated at 4°C storage conditions. Insonated clips achieved average cumulative VAN release of 101.8 ± 2.8% (81.4 ± 2.8 mg) after 72 hours. Uninsonated clips had only 0.3 ± 0.1% (0.3 ± 0.1 mg) average cumulative VAN release (P < .0001). Clips retrieved from the rabbit did not rupture with insonation nor produce MeB staining of surrounding tissues. In the pig, the PLA film was visibly ruptured and MeB tissue was observed following insonation, whereas the uninsonated clip was intact.
These results demonstrate ultrasound-triggered release of an encapsulated prophylactic solution and provide an important proof-of-concept for continuing large animal evaluations for translational merit.
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Details
- Title
- In Vitro and In Vivo Evaluation of Ultrasound-Triggered Release From Novel Spinal Device
- Creators
- Lauren J Delaney - Thomas Jefferson UniversitySelin Isguven - Thomas Jefferson UniversityRachel Hilliard - University of PennsylvaniaQuezia Lacerda - Drexel UniversityBrian E Oeffinger - Drexel UniversityPriscilla Machado - Thomas Jefferson UniversityThomas P Schaer - University of PennsylvaniaNoreen J Hickok - Thomas Jefferson UniversitySteven M Kurtz - Drexel UniversityFlemming Forsberg - Thomas Jefferson University
- Publication Details
- Journal of ultrasound in medicine
- Publisher
- American Institute of Ultrasound in Medicine (AIUM)
- Number of pages
- 12
- Grant note
- K99 AR078354 / NIAMS NIH HHS R01 AR069119 / NIAMS NIH HHS F32 AR072491 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000999287300001
- Scopus ID
- 2-s2.0-85161308489
- Other Identifier
- 991020581471504721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Acoustics
- Radiology, Nuclear Medicine & Medical Imaging