Journal article
In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization
Journal of neurocytology, v 17(5), pp 671-681
Oct 1988
PMID: 2905386
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Abstract
In situ hybridization histochemistry was used to identify neurons in rat dorsal root ganglia that contained mRNAs encoding β-preprotachykinin and preprosomatostatin. The distribution of these neurons was compared with the distribution of neurons containing tachykinins or somatostatin, identified using immunocytochemical techniques. Neurons labelled for β-preprotachykinin mRNA constituted 20% of the total neuronal population and belonged to the small cell class. Neurons labelled for preprosomatostatin mRNA with either RNA or DNA hybridization probes constituted approximately 10% of the total cells and comprised a small cell group that differed in average size from the β-preprotachykinin labelled population. The distribution of cells containing tachykinin- or somatostatin-like immunoreactive material was identical to the distribution of cells containing the respective mRNAs and, in addition, individual somata in adjacent sections contained both the mRNA precursor and the peptide. These results suggest that for these neuropeptides the sensitivity of the two methods is equivalent and the respective mRNAs and peptides are co-localized in the same neurons.
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Details
- Title
- In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization
- Creators
- Deborah B. HenkenAlan TesslerMarie-Françoise Chesselet - Drexel UniversityAlan HudsonFrank Baldino - Drexel UniversityMarion Murray
- Publication Details
- Journal of neurocytology, v 17(5), pp 671-681
- Publisher
- Springer Nature
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1988Q972400007
- Scopus ID
- 2-s2.0-0023724994
- Other Identifier
- 991019184026904721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Neurosciences