Journal article
In situ, in-liquid, all-electrical detection of Salmonella typhimurium using lead titanate zirconate/gold-coated glass cantilevers at any dipping depth
Biosensors & bioelectronics, v 22(12), pp 3132-3138
15 Jun 2007
PMID: 17387007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Most biosensing techniques are indirect, slow, and require labeling. Even though silicon-based microcantilever sensors are sensitive and label-free, they are not suitable for in-liquid detection. More recently lead zirconate titanate (PZT) thin-film-based microcantilevers are shown to be sensitive and in situ. However, they require microfabrication and must be electrically insulated. In this study, we show that highly sensitive, in situ, Salmonella typhimurium detection can be achieved at 90% relative humidity using a lead zirconate titanate (PZT)/gold-coated glass cantilever 0.7mm long with a non-piezoelectric 2.7mm long gold-coated glass tip by partially dipping the gold-coated glass tip in the suspension at any depth without electrically insulating the PZT. In particular, we showed that at 90% relative humidity and with a dipping depth larger than 0.8mm the PZT/gold-coated glass cantilever showed virtually no background resonance frequency up-shift due to water evaporation and exhibited a mass detection sensitivity of Δm/Δf=−5×10−11g/Hz. The concentration sensitivities of this PZT/gold-coated glass cantilever were 1×103 and 500cells/ml in 2ml of liquid with a 1 and 1.5mm dipping depth, respectively, both more than two orders of magnitude lower than the infectious dose and more than one order of magnitude lower than the detection limit of a commercial Raptor sensor.
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Details
- Title
- In situ, in-liquid, all-electrical detection of Salmonella typhimurium using lead titanate zirconate/gold-coated glass cantilevers at any dipping depth
- Creators
- Qing Zhu - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United StatesWan Y Shih - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, United StatesWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Biosensors & bioelectronics, v 22(12), pp 3132-3138
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000247555300053
- Scopus ID
- 2-s2.0-34248534560
- Other Identifier
- 991014877803004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology