Journal article
In situ quantification of aberrant p53 in colorectal neoplasia
Biomarkers, v 8(3-4), pp 311-332
2003
PMID: 12944180
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Aberrant p53 protein accumulation was measured immunohistologically in 342 colorectal paraffin-embedded tissue sections from 115 patients (24 with adenocarcinoma, 59 with adenoma and 32 'hospital controls'). Subjective scoring was compared with quantitative cell imaging, including dichotomous (p53+/p53−) status, ng p53mut mg−1 enterocyte protein, and tumour burden and patient body 'burden' of aberrant p53. A total of 62.5% cancer patients, 23.7% adenoma patients and 3.1% hospital controls were accorded p53+ status on the basis of p53 quantification. Quantitative p53+/p53− assignment had a stronger inverse association with survival (χ2=6.17, p=0.013, Kaplan-Meier test) than subjective 'visual estimation' (χ2=0.57, p=0.449). There was a strong inverse relationship between the p53 'body burden' and the months of post-diagnosis survival (hazard ratio=1.42, p=0.0004, Cox proportional hazards). Absolute quantification for inactivated p53 permits objective and reproducible scoring, adjusts for intra-laboratory immunostaining 'batch effects', corrects for fixation artefacts, and standardizes for inter-laboratory differences in fixation, antibody selection and staining method. Clinically, in situ quantification of p53 will permit more accurate survival prognoses and will inform therapy selection and dose. Ultimately, accurate quantitative tissue/blood p53 correlations may provide a minimally invasive and systemic surrogate measure for these same clinical purposes.
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Details
- Title
- In situ quantification of aberrant p53 in colorectal neoplasia
- Creators
- Steven Jay Smith - VA NY Harbor Healthcare SystemAlfred Neugut - Presbyterian Medical CenterDaniel Heitjan - Columbia UniversityKenneth Forde - NewYork–Presbyterian HospitalPeter Holt - St. Luke's-Roosevelt Hospital CenterRegina M. Santella - Columbia UniversityLuo Jiin-chyuan - National Taiwan UniversityWalter Carney - Bayer (United States)Llewelyn Ward - NewYork–Presbyterian HospitalPaul W. Brandt-rauf - Columbia University
- Publication Details
- Biomarkers, v 8(3-4), pp 311-332
- Publisher
- Informa UK Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000184952000010
- Scopus ID
- 2-s2.0-12444262322
- Other Identifier
- 991019323675804721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Toxicology