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Accepted (AM)Open Access (License Unspecified), Open
Journal article
In vitro gene delivery with ultrasound-triggered polymer microbubbles
Ultrasound in medicine & biology, v 39(6), pp 1102-1119
Jun 2013
PMID: 23562023
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In the work described here, gene delivery using polymer microbubbles triggered by ultrasound in vitro was investigated. The effects of pressure amplitude (0-2 MPa), center frequency (1-5 MHz), pulse length (3-12,000 μs), pulse repetition frequency (5-20,000 Hz) and exposure time (0-30 s) on transfection efficiency and cell viability were examined. The effects of radiation force, calcium ion concentration and timing of treatments were also examined. Cells were successfully transfected with pressure amplitudes as low as 250 kPa. Transfection was most efficient at lower frequencies and longer pulse lengths, with a transfection efficiency of 24.2 ± 2.0% achieved using a center frequency of 1 MHz, pressure amplitude of 1 MPa, pulse length of 12,000 μs and pulse repetition frequency of 5 Hz. Gene delivery was also affected by the extracellular calcium ion concentration and the timing of treatments.
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Details
- Title
- In vitro gene delivery with ultrasound-triggered polymer microbubbles
- Creators
- Michael Cochran - Drexel UniversityMargaret A Wheatley - Drexel University
- Publication Details
- Ultrasound in medicine & biology, v 39(6), pp 1102-1119
- Publisher
- Elsevier
- Grant note
- R01 HL052901 / NHLBI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000319260000020
- Scopus ID
- 2-s2.0-84877156583
- Other Identifier
- 991019168231504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Acoustics
- Radiology, Nuclear Medicine & Medical Imaging