Journal article
In vitro labeling of gonadal steroid hormone receptors in brain tissue sections
Steroids, v 60(11), pp 726-737
01 Nov 1995
PMID: 8585096
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Autoradiographic methods have been developed for measurement of gonadal steroid receptors in situ in brain tissue sections. Based on principles established previously for estrogen receptors in the rat brain using a
125I-labeled ligand, procedures have been developed for in vitro labeling of estrogen, androgen, and progestin receptors with commercially available tritiated ligands. Addition of protamine sulfate to the incubation buffer precipitates the receptors in situ in the tissue sections, allowing them to be detected autoradiographically after incubation with labeled steroid and subsequent washing to remove unbound and nonspecifically bound ligand. Occupied and unoccupied estrogen receptors can be measured selectively using appropriately modified incubation conditions. In the case of androgen and progestin receptors, unoccupied receptors are readily detected by in vitro labeling of tissue sections, but occupied receptors do not appear to label efficiently. Preliminary data suggest that these methods should be equally applicable to a variety of laboratory animals, including the rat, mouse, guinea pig, and monkey.
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Details
- Title
- In vitro labeling of gonadal steroid hormone receptors in brain tissue sections
- Creators
- Theodore J. Brown - University of TorontoMonika Sharma - University of TorontoLawrence E. Heisler - University of TorontoNaznin Karsan - University of TorontoMichael J. Walters - Imaging Research, Inc. St. Catharines, Ontario, CanadaNeil J. MacLusky - University of Toronto
- Publication Details
- Steroids, v 60(11), pp 726-737
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:A1995TA66400001
- Scopus ID
- 2-s2.0-0028886690
- Other Identifier
- 991020950598204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism