Journal article
In vivo and in vitro anti-cancer activity of thermo-sensitive and photo-crosslinkable doxorubicin hydrogels composed of chitosan–doxorubicin conjugates
European journal of pharmaceutics and biopharmaceutics, v 73(1), pp 59-65
01 Sep 2009
PMID: 19409990
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Doxorubicin was chemically conjugated to acrylated chitosan in order to obtain sustained-release profiles of doxorubicin from thermo-responsive and photo-crosslinkable hydrogels. Chitooligosaccharide was acrylated with glycidyl methacrylate and subsequently conjugated to doxorubicin via an amide linkage. A mixture of doxorubicin–chitosan conjugates, acrylated Pluronic, and doxorubicin formed physical gels at 37
°C. Photo-irradiation was subsequently performed to chemically crosslink the physical hydrogel at 37
°C. Chitooligosaccharide–doxorubicin conjugates in the doxorubicin hydrogels significantly reduced burst release of free doxorubicin from doxorubicin hydrogels compared hydrogels without the conjugates. Upon incubating doxorubicin hydrogels at 37
°C, chitosan–doxorubicin conjugates were confirmed to be degraded into more hydrophilic oligomers by reversed-phase chromatography.
In vitro cytotoxicity assay using released media from doxorubicin hydrogels showed that degraded chitosan–doxorubicin had cytotoxicity comparable to free doxorubicin. Athymic nude mice bearing human lung adenocarcinoma were subjected to intra-tumoral injections of physical hydrogels. After photo-crosslinking injected hydrogels using surgical catheters, tumor sizes, body weights, and survivals were measured for 1
month. Released media from doxorubicin hydrogels exerted similar cytotoxicities to free doxorubicin, and the tumor volume was significantly reduced for 1
month compared to other samples. Thus, doxorubicin hydrogels containing doxorubicin conjugates can be employed as a novel injectable anti-cancer drug aiming to achieve sustained release of doxorubicin for several weeks against solid tumors.
Metrics
Details
- Title
- In vivo and in vitro anti-cancer activity of thermo-sensitive and photo-crosslinkable doxorubicin hydrogels composed of chitosan–doxorubicin conjugates
- Creators
- Young Il Cho - Kyung Hee UniversityShinyoung Park - Kangwon National UniversitySeo Young Jeong - Kyung Hee UniversityHyuk Sang Yoo - Kangwon National University
- Publication Details
- European journal of pharmaceutics and biopharmaceutics, v 73(1), pp 59-65
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000270016200007
- Scopus ID
- 2-s2.0-68349158961
- Other Identifier
- 991020547324304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Pharmacology & Pharmacy